Results 51 to 60 of about 532,169 (312)

Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein.

, 2020
RNA-binding proteins (RBPs) play important roles in bacterial gene expression and physiology but their true number and functional scope remain little understood even in model microbes.
Vogel, Jörg, Barquist, Lars, El Mouali, Youssef, Kuper, Jochen, Kisker, Caroline, Gerovac, Milan   +5 more
core   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Systematic identification of bacterial factors driving Staphylococcus aureus intracellular lifestyle in non-professional phagocytes

Nature Communications
Staphylococcus aureus is a major human pathogen responsible for severe infections. While traditionally described as extracellular, increasing evidence establishes S. aureus as a facultative intracellular pathogen.
Ines Rodrigues Lopes, Laura Maria Alcantara, Maria Lopez-Bravo, Yi Liu, Gerald Larrouy-Maumus, Daniel Lopez, Miguel Mano, Ana Eulalio   +7 more
doaj   +1 more source

Localization of the Bacterial RNA Infrastructure [PDF]

, 2011
The bacterial RNA network includes most of the same components found in eukaryotes, and many of the interactions that under lie transcription, RNA processing and stability, translation, and protein secretion are conserved. The major difference is that all of these functions take place in a single cellular compartment.
openaire   +2 more sources

Conformational flexibility of bacterial RNA polymerase [PDF]

Proceedings of the National Academy of Sciences, 2002
The structure of Escherichia coli core RNA polymerase (RNAP) was determined by cryo-electron microscopy and image processing of helical crystals to a nominal resolution of 15 Å. Because of the high sequence conservation between the core RNAP subunits, we were able to interpret the E.
Seth A, Darst, Natacha, Opalka, Pablo, Chacon, Andrey, Polyakov, Catherine, Richter, Gongyi, Zhang, Willy, Wriggers   +6 more
openaire   +2 more sources

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Accurately assembling nanopore sequencing data of highly pathogenic bacteria

BMC Genomics
Background Bacterial genome exploration and outbreak analysis rely heavily on robust whole-genome sequencing and bioinformatics analysis. Widely-used genomic methods, such as genotyping and detection of genetic markers demand high sequencing accuracy and
Christine Thomas, Hanka Brangsch, Valentina Galeone, Martin Hölzer, Manja Marz, Jörg Linde   +5 more
doaj   +1 more source

Novel Peptide Conjugates of Modified Oligonucleotides for Inhibition of Bacterial RNase P

Frontiers in Pharmacology, 2019
Novel alternatives to traditional antibiotics are now of great demand for the successful treatment of microbial infections. Here, we present the engineering and properties of new oligonucleotide inhibitors of RNase P, an essential bacterial enzyme.
Darya Novopashina, Darya Novopashina, Mariya Vorobyeva, Anton Nazarov, Anna Davydova, Nikolay Danilin, Lyudmila Koroleva, Lyudmila Koroleva, Andrey Matveev, Alevtina Bardasheva, Nina Tikunova, Nina Tikunova, Maxim Kupryushkin, Dmitrii Pyshnyi, Dmitrii Pyshnyi, Sidney Altman, Sidney Altman, Alya Venyaminova   +17 more
doaj   +1 more source

The Phage-Encoded N-Acetyltransferase Rac Mediates Inactivation of Pseudomonas aeruginosa Transcription by Cleavage of the RNA Polymerase Alpha Subunit

Viruses, 2020
In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses.
Pieter-Jan Ceyssens, Jeroen De Smet, Jeroen Wagemans, Natalia Akulenko, Evgeny Klimuk, Subray Hedge, Marleen Voet, Hanne Hendrix, Jan Paeshuyse, Bart Landuyt, Hua Xu, John Blanchard, Konstantin Severinov, Rob Lavigne   +13 more
doaj   +1 more source

Study of E. coli Hfq's RNA annealing acceleration and duplex destabilization activities using substrates with different GC-contents

, 2012
Folding of RNA molecules into their functional three-dimensional structures is often supported by RNA chaperones, some of which can catalyse the two elementary reactions helix disruption and helix formation.
Saxena, Krishna, Doetsch, Martina, Stampfl, Sabine, Beich-Frandsen, Mads, Lybecker, Meghan, Fürtig, Boris, Schroeder, Renée   +6 more
core   +1 more source