Results 71 to 80 of about 532,169 (312)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

APEX2 proximity labeling of RNA in bacteria

Cell Reports: Methods
Summary: Rapid, spatially controlled methods are needed to investigate RNA localization in bacterial cells. APEX2 proximity labeling was shown to be adaptable to rapid RNA labeling in eukaryotic cells and, through the fusion of APEX2 to different ...
Hadi Yassine, Elizabeta Sirotkin, Omer Goldberger, Vincent A. Lawal, Daniel B. Kearns, Orna Amster-Choder, Jared M. Schrader   +6 more
doaj   +1 more source

Purification of Bacterial RNA from Infected Macrophages

Bio-Protocol, 2015
Studying the transcriptome of bacterial pathogens during infection is a very informative and effective tool for discovering genes that contribute to successful infection.
Lior Lobel, Nadejda Sigal, Anna Pasechnek, Anat Herskovits   +3 more
doaj   +1 more source

Effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (Solanum tuberosum)

, 2010
Beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization.
Overbeek, L.S., van, Azevedo, J.L., Andreote, F.D., Rocha, U.N., da, Araujo, W.L.   +4 more
core   +1 more source

The bacterial enhancer-dependent RNA polymerase [PDF]

Biochemical Journal, 2016
Transcription initiation is highly regulated in bacterial cells, allowing adaptive gene regulation in response to environment cues. One class of promoter specificity factor called sigma54 enables such adaptive gene expression through its ability to lock the RNA polymerase down into a state unable to melt out promoter DNA for transcription initiation ...
Zhang, N, Darbari, VC, Glyde, R, Zhang, X, Buck, M   +4 more
openaire   +3 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Assessment of bacterial and viral gut communities in healthy and tumoral colorectal tissue using RNA and DNA deep sequencing

Cancer Medicine, 2023
Background Colorectal cancer (CRC) is known to present a distinct microbiome profile compared to healthy mucosa. Non‐targeted deep‐sequencing strategies enable nowadays full microbiome characterization up to species level.
Ainhoa Garcia‐Serrano, Dhananjay Mukhedkar, Emilie Hultin, Ulla Rudsander, Yvonne Wettergren, Agustín Enrique Ure, Joakim Dillner, Laila Sara Arroyo‐Mühr   +7 more
doaj   +1 more source

A computational strategy for the search of regulatory small RNAs in Actinobacillus pleuropneumoniae. [PDF]

, 2016
Bacterial regulatory small RNAs (sRNAs) play important roles in gene regulation and are frequently connected to the expression of virulence factors in diverse bacteria.
Bossé, JT, Witney, AA, Langford, Paul R., Li, Yanwen, Gould, Kate A., Witney, Adam A., Rossi, CC, Bazzolli, Denise M.S., Bazzolli, DMS, Li, Y, Gould, KA, Bossé, Janine T., Langford, PR, Rossi, Ciro C.   +13 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source