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Rifampin mediated decapsulation and enhanced bacterial clearance: a new mechanism of rifamycins. [PDF]
EBioMedicineLee GY, Song J.
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16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020-2023. [PDF]
Emerg Infect DisLi G +9 more
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<i>In vitro</i> impact of <i>Streptococcus mitis</i> on the inhibition of oral cancer cell proliferation via mitotic modulation. [PDF]
Front Cell Infect MicrobiolInui I +8 more
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Current Protocols in Molecular Biology, 1993
AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
Michael Z. Gilman, K.J. Reddy
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AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
Michael Z. Gilman, K.J. Reddy
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Autodegradation of RNA of bacterial ribosomes
Canadian Journal of Microbiology, 1973The autodegradation capacity of ribosomes, in the presence or absence of EDTA, has been studied in resting and dividing bacteria. This capacity, in the presence of EDTA, was found to be much lower in the ribosomes of log-phase bacteria when compared to stationary phase bacteria. In absence of EDTA, autodegradation was not observed.
C. Gagnon, G. de Lamirande
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Cold Spring Harbor Protocols, 2012
In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
openaire +3 more sources