Results 81 to 90 of about 477,009 (248)

Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

Nature Communications, 2020
Bacterial transcription is terminated when RNA polymerases encounter terminator sequences. Using a single-molecule fluorescence assay, here the authors show that the release of transcript RNA precedes RNA polymerase dissociation and that the remaining ...
Wooyoung Kang, Kook Sun Ha, Heesoo Uhm, Kyuhyong Park, Ja Yil Lee, Sungchul Hohng, Changwon Kang   +6 more
doaj   +1 more source

Estimating the Postmortem Interval of Carcasses in the Water Using the Carrion Insect, Brain Tissue RNA, Bacterial Biofilm, and Algae. [PDF]

Front Microbiol, 2021
Wang Y, Wang M, Xu W, Wang Y, Zhang Y, Wang J.   +5 more
europepmc   +1 more source

Biomaterial Strategies for Targeted Intracellular Delivery to Phagocytes

Advanced Functional Materials, EarlyView.
Phagocytes are essential to a functional immune system, and their behavior defines disease outcomes. Engineered particles offer a strategic opportunity to target phagocytes, harnessing inflammatory modulation in disease. By tuning features like size, shape, and surface, these systems can modulate immune responses and improve targeted treatment for a ...
Kaitlyn E. Woodworth, Neal I. Callaghan, Locke Davenport Huyer   +2 more
wiley   +1 more source

Next‐Generation Bio‐Reducible Lipids Enable Enhanced Vaccine Efficacy in Malaria and Primate Models

Advanced Functional Materials, EarlyView.
Structure–activity relationship (SAR) optimization of bio‐reducible ionizable lipids enables the development of highly effective lipid nanoparticle (LNP) mRNA vaccines. Lead LNPs show superior tolerability and antibody responses in rodents and primates, outperforming approved COVID‐19 vaccine lipids.
Ruben De Coen, Sabah Kasmi, Shrinivas Dumbre, Lies Baekens, Elise Seynaeve, Louise Van den Hauwe, Niccolò Cantini, Aello Garantoudi, Filipe Branco Madeira, Alain Marrot, Emma Tuypens, Sofie Seghers, Anna Kuchmiy, Elisabeth Brabants, Bart Vanderborght, Ine Maes, Sarah Vandenberghe, Jessica Filtjens, Alícia Gordún Peiró, Daliya Kancheva, Ayla Debraekeleer, Florence Lambolez, Emily De Lombaerde, Yong Chen, Rafael Miyazawa Martins, Pauline Formaglio, Damya Laoui, Sofie Deschoemaeker, Bruno G. De Geest, Rogerio Amino, Stefaan De Koker   +30 more
wiley   +1 more source

Metatranscriptomic profiling reveals pathogen and host response signatures of pediatric acute sinusitis and upper respiratory infection

Genome Medicine
Background Acute sinusitis (AS) is a frequent cause of antibiotic prescriptions in children. Distinguishing bacterial AS from common viral upper respiratory infections (URIs) is crucial to prevent unnecessary antibiotic use but is challenging with ...
Andrew C. Doxey, Nooran Abu Mazen, Max Homm, Vivian Chu, Manjot Hunjan, Briallen Lobb, Sojin Lee, Marcia Kurs-Lasky, John V. Williams, William MacDonald, Monika Johnson, Jeremy A. Hirota, Nader Shaikh   +12 more
doaj   +1 more source

An optimised saliva collection method to produce high-yield, high-quality RNA for translational research.

PLoS ONE, 2020
Saliva represents an ideal matrix for diagnostic biomarker development as it is readily available and requires no invasive collection procedures. However, salivary RNA is labile and rapidly degrades.
Roisin Sullivan, Susan Heavey, David G Graham, Rachel Wellman, Saif Khan, Sri Thrumurthy, Benjamin S Simpson, Tina Baker, Sarah Jevons, Jose Ariza, Victor Eneh, Hayley Pye, Hayley Luxton, Rifat Hamoudi, Hayley Whitaker, Laurence B Lovat   +15 more
doaj   +1 more source

DENTA: A Dual Enzymatic Nanoagent for Self‐Activating Tooth Whitening and Biofilm Disruption

Advanced Functional Materials, EarlyView.
The nanoapatite with dual enzymes (DENTA) accumulates in dentinal tubules, reducing hypersensitivity caused by dental nerve exposure and facilitating continuous ROS generation through salivary glucose for effective, long‐term whitening. The dentin structures remain non‐destructive due to the low concentration of ROS, demonstrating excellent cell ...
Junseok Kim, Dai‐Hwan Kim, Priyannth R. Sundharbaabu, Chae Yeon Lee, Jina Bae, Jiyu Hyun, Young‐Ju Jang, Haeni Kim, Min‐Ho Hong, Juewen Liu, Tobias Fey, Suk Ho Bhang, Jun Hyuk Heo, Jung Heon Lee   +13 more
wiley   +1 more source

Modular Architecture of Bacterial RNase P Ribozymes as a Structural Platform for RNA Nanostructure Design

CHIMIA, 2018
Ribonuclease P (RNase P) is a class of enzymes involved in the processing of precursor tRNAs to remove their 5'-leader sequences. Ribonuclease P enzymes are classified into two completely distinct classes, i.e.
Yuri Nozawa, Megumi Hagihara, Shigeyoshi Matsumura, Yoshiya Ikawa   +3 more
doaj   +1 more source

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Enhanced Sonodynamic Bacterial Elimination and Wound Healing Therapy Based on Lanthanide Ion Doped Bi2WO6 Nanosheets and Hydrogel Platform

Advanced Functional Materials, EarlyView.
Lanthanide ytterbium ions are introduced into Bi2WO6 nanosheets to boost ROS generation under US stimulation. These ytterbium‐doped Bi2WO6 nanosheets serve as efficient sonosensitizers, enabling broad‐spectrum bacterial eradication. To improve biocompatibility for in vivo sonodynamic therapy, the sonosensitizers are integrated into hydrogel matrix ...
Xinyue Lao, Qianqian Bai, Yifei Zhao, Xingyi Dai, Yuan Liu, Xiao Han, Jianhua Hao   +6 more
wiley   +1 more source