Nucleic Acids Research, 2012 16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth +6 more
semanticscholar +1 more source
Conformational flexibility of bacterial RNA polymerase [PDF]
Proceedings of the National Academy of Sciences, 2002 The structure of Escherichia coli core RNA polymerase (RNAP) was determined by cryo-electron microscopy and image processing of helical crystals to a nominal resolution of 15 Å. Because of the high sequence conservation between the core RNAP subunits, we were able to interpret the E.
Seth A, Darst +6 more
openaire +2 more sources
Highly efficient 5\u27 capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase [PDF]
, 2018 Bacterial and eukaryotic nuclear RNA polymerases (RNAPs) cap RNA with the oxidized and reduced forms of the metabolic effector nicotinamide adenine dinucleotide, NAD+ and NADH, using NAD+ and NADH as non-canonical initiating nucleotides for transcription
Basu, Urmimala +11 more
core +1 more source
In vivo analysis of staphylococcus aureus-infected mice reveals differential temporal and spatial expression patterns of fhuD2 [PDF]
, 2017 Staphylococcus aureus is an opportunistic human pathogen and a major cause of invasive infections such as bacteremia, endocarditis, pneumonia and wound infections.
Bacconi, Marta +7 more
core +1 more source
Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity
FEMS Microbiology Reviews, 2015 CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series
E. Charpentier +3 more
semanticscholar +1 more source
Bacterial small RNAs in the Genus Rickettsia [PDF]
BMC Genomics, 2015 Rickettsia species are obligate intracellular Gram-negative pathogenic bacteria and the etiologic agents of diseases such as Rocky Mountain spotted fever (RMSF), Mediterranean spotted fever, epidemic typhus, and murine typhus. Genome sequencing revealed that R. prowazekii has ~25 % non-coding DNA, the majority of which is thought to be either "junk DNA"
Casey L. C. Schroeder +8 more
openaire +2 more sources
A link between transcription fidelity and pausing in vivo [PDF]
, 2017 Pausing by RNA polymerase is a major mechanism that regulates transcription elongation but can cause conflicts with fellow RNA polymerases and other cellular machineries. Here, we summarize our recent finding that misincorporation could be a major source
Gamba, Pamela +2 more
core +2 more sources
Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release. [PDF]
, 2018 Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A site and is mediated by class I release factors (RFs).
Clementi, Nina +11 more
core +3 more sources
Membrane vesicle-mediated release of bacterial RNA
Scientific Reports, 2015 Many Gram-negative bacterial species release outer membrane vesicles (OMVs) that interact with the host by delivering virulence factors. Here, we report for the first time that RNA is among the wide variety of bacterial components that are associated ...
Annika E. Sjöström +3 more
semanticscholar +1 more source
RNA-Binding Proteins Driving the Regulatory Activity of Small Non-coding RNAs in Bacteria
Frontiers in Molecular Biosciences, 2020 Small non-coding RNAs (sRNAs) are critical post-transcriptional regulators of gene expression. Distinct RNA-binding proteins (RBPs) influence the processing, stability and activity of bacterial small RNAs.
Ana P. Quendera +6 more
doaj +1 more source