Results 61 to 70 of about 2,383,057 (293)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA [PDF]

Journal of Virology, 1988
X double-stranded RNA (dsRNA) is a 0.52-kilobase dsRNA molecule that arose spontaneously in a nonkiller strain of Saccharomyces cerevisiae originally containing L-A and L-BC dsRNAs (L-BC is the same size as L-A but shares no homology with it). X hybridized with L-A, and direct RNA sequencing of X showed that the first 5' 25 base pairs (of the X ...
R, Esteban, R B, Wickner
openaire   +2 more sources

‘Guide and Prejudice’— How Argonautes recognize targets across domains of life

FEBS Letters, EarlyView.
Argonaute proteins use short nucleic‐acid guides to locate and regulate specific targets across all domains of life. Despite striking diversity—from human gene silencing to bacterial immune defence—all Argonautes share a conserved three‐stage recognition logic: guide‐directed sampling, progressive target pairing with a conformational checkpoint and ...
Jack P. K. Bravo
wiley   +1 more source

Engineering double-stranded RNA binding activity into the Drosha double-stranded RNA binding domain results in a loss of microRNA processing function.

PLoS ONE, 2017
Canonical processing of miRNA begins in the nucleus with the Microprocessor complex, which is minimally composed of the RNase III enzyme Drosha and two copies of its cofactor protein DGCR8.
Joshua C Kranick, Durga M Chadalavada, Debashish Sahu, Scott A Showalter   +3 more
doaj   +1 more source

Characterization of Supercoiled Double-Stranded RNA, and Comparison to Double-Stranded DNA [PDF]

Biophysical Journal, 2011
While the mechanical properties of double-stranded DNA have been intensely studied using various single molecule assays, much less is known about double-stranded RNA (dsRNA). dsRNA is important in Biology as it forms the hereditary material for a number of viruses, and is also central to the RNA mediated gene-silencing pathway.
Skinner, Gary M., Donkers, Serge P., Lipfert, Jan, Huang, Zhuangxiong, Dekker, Nynke H.   +4 more
openaire   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

TRBP and eIF6 homologue in Marsupenaeus japonicus play crucial roles in antiviral response.

PLoS ONE, 2012
Plants and invertebrates can suppress viral infection through RNA silencing, mediated by RNA-induced silencing complex (RISC). Trans-activation response RNA-binding protein (TRBP), consisting of three double-stranded RNA-binding domains, is a component ...
Shuai Wang, An-Jing Chen, Li-Jie Shi, Xiao-Fan Zhao, Jin-Xing Wang   +4 more
doaj   +1 more source

Tomato chlorosis virus CPm protein is a pathogenicity determinant and suppresses host local RNA silencing induced by single-stranded RNA

Frontiers in Microbiology, 2023
IntroductionTomato chlorosis virus (ToCV) is a typical member of the genus Crinivirus, which severely threatens Solanaceae crops worldwide. The CPm protein encoded by ToCV has been reported to be associated with virus transmission by vectors and is ...
Xiao Yang, Xiangwen Luo, Xiangwen Luo, Yu Zhang, Zhanhong Zhang, Zhanhong Zhang, Xian OuYang, Xiaobin Shi, Xiaobin Shi, Xiaoyuan Lv, Fan Li, Songbai Zhang, Songbai Zhang, Yong Liu, Yong Liu, Deyong Zhang, Deyong Zhang   +16 more
doaj   +1 more source

Presence of double-stranded RNAs in human and calf isolates of Cryptosporidium parvum [PDF]

, 2000
We examined the occurrence of 2 virus-like double-stranded (ds)RNAs in human and calf isolates of Cryptosporidium parvum senso latu and other microorganisms, including 7 other members of the genus. A total of 32 isolates of C.
Dykstra, C.C., Morgan, U.M., Khramtsovk, N.V., Griffiths, J.K., Upton, S.J., Chung, P.A., Arrowood, M.J.   +6 more
core  

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source