Results 41 to 50 of about 384,029 (308)

Double-Stranded RNA Sensors and Modulators in Innate Immunity

Annual Review of Immunology, 2019
Detection of double-stranded RNAs (dsRNAs) is a central mechanism of innate immune defense in many organisms. We here discuss several families of dsRNA-binding proteins involved in mammalian antiviral innate immunity.
S. Hur
semanticscholar   +1 more source

High-Pressure-Sprayed Double Stranded RNA Does Not Induce RNA Interference of a Reporter Gene

Frontiers in Plant Science, 2020
In plants, RNA interference (RNAi) is an effective defense mechanism against pathogens and pests. RNAi mainly involves the micro RNA and the small interfering RNA (siRNA) pathways.
Veli Vural Uslu, Alexandra Bassler, Gabi Krczal, Michael Wassenegger, Michael Wassenegger   +4 more
doaj   +1 more source

Comparative analysis of double-stranded RNA degradation and processing in insects

Scientific Reports, 2017
RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon.
I. Singh, Satnam Singh, Kanakachari Mogilicherla, J. Shukla, S. R. Palli   +4 more
semanticscholar   +1 more source

Stress Granules Regulate Double-Stranded RNA-Dependent Protein Kinase Activation through a Complex Containing G3BP1 and Caprin1

mBio, 2015
Stress granules (SGs) are dynamic cytoplasmic repositories containing translationally silenced mRNAs that assemble upon cellular stress. We recently reported that the SG nucleating protein G3BP1 promotes antiviral activity and is essential in double ...
Lucas C. Reineke, N. Kedersha, M. Langereis, F. V. van Kuppeveld, R. Lloyd   +4 more
semanticscholar   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans

Genome Biology, 2000
BackgroundIn Caenorhabditis elegans, injection of double-stranded RNA (dsRNA) results in the specific inactivation of genes containing homologous sequences, a technique termed RNA-mediated interference (RNAi).
R. Kamath, Maruxa Martínez‐Campos, Peder Zipperlen, A. Fraser, J. Ahringer   +4 more
semanticscholar   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Supercoiling Double-Stranded RNA [PDF]

Biophysical Journal, 2010
Through a novel "polymerase-stall" labeling procedure, we have successfully generated torsionally constrained molecules of double-stranded RNA (dsRNA). We have anchored these molecules within a magnetic tweezers apparatus, and by rotating the magnets, induced both positive and negative supercoils within these molecules.
Skinner, Gary M., Donkers, Serge P., Lipfert, Jan, Dekker, Nynke H.   +3 more
openaire   +1 more source