Results 171 to 180 of about 133,666 (284)

Model‐Guided Systematic Metabolic Engineering for Enhanced Spinosad Biosynthesis in Saccharopolyspora spinosa NHF132

Advanced Science, EarlyView.
A genome‐scale model guided the systematic metabolic engineering of Saccharopolyspora spinosa, enabling a 5.5‐fold boost in the green bioinsecticide spinosad to 1.8 g L−1 and establishing an actinomycete framework for complex natural products overproduction.
Shuliu Wang, Yuxin Liu, Qian Zhang, Yue Jiang, Xiaoqian Zeng, Chengyu Zhang, Jiagao Cheng, Weishan Wang, Lixin Zhang, Gao‐Yi Tan   +9 more
wiley   +1 more source

Next Generation Sequencing Improves Diagnostic 16S rRNA Amplicon-Based Microbiota Analyses of Clinical Samples Compared to Sanger Sequencing. [PDF]

APMIS
Aftab H, Schouw CH, Dargis R, Vejrum LK, Johansen RL, Møller JT, Madsen TV, Laugesen AL, Christensen JJ, Kemp M, Nielsen XC.   +10 more
europepmc   +1 more source

Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research.

Journal of Investigative Dermatology, 2016
J. Jo, Elizabeth A. Kennedy, H. Kong
semanticscholar   +1 more source

CRISPR‐DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution

Advanced Science, EarlyView.
This study develops an in vivo targeted mutagenesis system based on CRISPR‐guided bacteriophage T5 or T7 DNA polymerases. This system can enable all possible types of substitution mutations within an expanded mutagenesis window in Escherichia coli and exhibit application potential in different microorganisms, thus accelerating the evolution of ...
Shuaili Chen, Xiangdi Chen, Yifan Peng, Qinghua Li, Jingwen Zhou, Jianghua Li, Guocheng Du, Jian Chen, Guoqiang Zhang   +8 more
wiley   +1 more source

Unveiling Novel Viral Diversity, Biogeography, and Host Networks in Wildlife Through High‐Throughput Sequencing Data Mining

Advanced Science, EarlyView.
Analysis of 57 536 high‐throughput sequencing datasets uncovers a vast, hidden world of viruses in wildlife. The researchers reveal significant geographic and host‐specific patterns of viruses, and their surprising cross‐species transmissions, such as avian flu viruses infecting goats.
Hai Wang, Yafei Meng, Xiaoyuan Chen, Xinyuan Cui, Qian Zuo, Na Han, Xianghui Liang, Xuejuan Shen, Caiwu Li, Desheng Li, Fumin Wang, Liangping He, Rujian Chen, Xingbang Lu, Wenjie You, Aiping Wu, Rui‐Ai Chen, Wu Chen, Chan Ding, Yongyi Shen   +19 more
wiley   +1 more source

Optimization of 16S RNA Sequencing and Evaluation of Metagenomic Analysis with Kraken 2 and KrakenUniq. [PDF]

Diagnostics (Basel)
Papa Mze N, Fernand-Laurent C, Maxence S, Zanzouri O, Daugabel S, Marque Juillet S.   +5 more
europepmc   +1 more source

RPS27L Enhances Myogenesis and Muscle Mass by Targeting IGF1 Through Liquid‐Liquid Phase Separation

Advanced Science, EarlyView.
Muscle‐specific RPS27L knock‐in mice exhibit increased body weight, muscle mass, and enhanced muscle regenerative capacity. RPS27L drives myogenesis by promoting myoblast proliferation, while inhibiting myogenic differentiation. Mechanistically, RPS27L targets IGF1 protein by liquid‐liquid phase separation through its N‐terminal intrinsically ...
Xiaoqin Liu, Yilong Yao, Junyu Yan, Mu Zeng, Xinhao Fan, Yijie Tang, Jiju Li, Yanwen Liu, Shanying Yan, Wei Wang, Lijuan Chen, Ruipu Chen, Yuxin Huang, Honor Calnan, Heng Wang, Graham Gardner, Yalan Yang, Zhonglin Tang   +17 more
wiley   +1 more source

16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020-2023. [PDF]

Emerg Infect Dis
Li G, Reis CA, Kruc RM, Zhang Z, Streck NT, Ristagno EH, Mandrekar J, Wolf MJ, Gaensbauer JT, Patel R.   +9 more
europepmc   +1 more source

30S Ribosomal proteins associated with the 3′‐terminus of 16S RNA [PDF]

FEBS Letters, 1975
Georg Stöffler, Charles G. Kurland, A.P. Czernilofsky   +2 more
openaire   +3 more sources

T‐2 Toxin Exploits Gut‐Derived Staphylococcus Saprophyticus to Disrupt Hepatic Macrophage Homeostasis

Advanced Science, EarlyView.
T‐2 toxin promotes the proliferation of intestinal S. saprophyticus and facilitates its translocation to the liver in piglets and mice. The translocated bacteria trigger autophagy in Kupffer cells and recruit monocytes to the liver through the nucleotide‐binding oligomerization domain 2 (NOD2) signaling pathway, alongside the M1 polarization of hepatic
Yuanyuan Zhu, Liu Xu, Fangrui Guo, Jianyu Qu, Xiangyan Liu, Qiurong Xu, Jie Sheng, Jiangping Wang, Xiaohong Xie, Ruimin Ren, Chuan Zhou, Sisi Yan, Shuiping Liu, Zhihang Yuan, Rongfang Li, Jing Wu, Jine Yi, Yulong Yin, Lixin Wen, Ji Wang   +19 more
wiley   +1 more source