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Nuclease S1 mapping of 16S ribosomal RNA in ribosomes

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1990
Escherichia coli 16S rRNA and 16S-like rRNAs from other species have several universally conserved sequences which are believed to be single-stranded in ribosomes. The quantitative disposition of these sequences within ribosomes is not known. Here we describe experiments designed to explore the availability of universal 16S rRNA sequences for ...
M A, Rahman, H W, Schaup
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Precursor 16S RNA in Active 30S Ribosomes

Nature, 1974
BIOGENESIS of bacterial ribosomes proceeds through the formation of precursor particles1–4, consisting of an incomplete set of ribosomal protein5 and of RNA molecules that are longer and less methylated than mature RNA4,6,7. RNA maturation is commonly considered to be a part of the ribosome maturation process.
MANGIAROTTI, Giorgio   +3 more
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Ribosomal activity of the 16 S · 23 S RNA complex

Archives of Biochemistry and Biophysics, 1985
It has been demonstrated in this laboratory that 16 S and 23 S RNAs form a binary complex like 30 S and 50 S ribosomes under certain specific conditions, and 5 S RNA can be incorporated into the complex in stoichiometric amounts in presence of three ribosomal proteins, L5, L18, and L15/25. These studies raised the basic question of whether such complex
D P, Burma, D S, Tewari, A K, Srivastava
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Secondary Structure of 16 S Ribosomal RNA

Science, 1981
A secondary structure model for 16 S ribosomal RNA which is based on available chemical, enzymatic, and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations.
H F, Noller, C R, Woese
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Nucleotide Sequences of Sections of 16S Ribosomal RNA

Nature, 1970
Three large sections of 16S RNA, obtained by partial enzymatic hydrolysis, and together representing nearly one-fifth of the molecule, have been analysed. Knowledge of their primary structure allows features of the secondary structure to be envisaged.
C, Ehresmann, P, Fellner, J P, Ebel
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Conservation of primary structure in 16S ribosomal RNA

Nature, 1975
THERE is no doubt that the large ribosomal RNAs play specific roles in ribosome function1. Yet the thrust of experimentation during the past 5 years indicates clearly that the biologist tends to view these roles as structural, function being reserved by and large for the ribosomal protein components2,3.
C R, Woese   +7 more
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Comparative Anatomy of 16-S-like Ribosomal RNA

1985
Publisher Summary This chapter examines the range of the variation of secondary structure among the 16-S-like rRNAs. This brings into a larger structural context a recent detailed analysis of the individual helical elements and provides a basis for an accurate alignment of the corresponding regions of different primary structures.
R R, Gutell   +3 more
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Chromosomal loci for 16S ribosomal RNA in Escherichia coli

Molecular and General Genetics MGG, 1972
Genetic loci for 16S ribosomal RNA (rRNA) on the Escherichia coli chromosome were determined using the K-sequence, a characteristic oligonucleotide of strain K12, as a genetic marker. Oligonucleotide analyses of 16S rRNA from various recombinants between strain K12 and strain B(H) showed that the loci for 16S rRNA containing the K-sequence were near ...
M, Matsubara, R, Takata, S, Osawa
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16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis

Free Radical Biology and Medicine, 1997
The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10 micromol H(2)O(2) per 10(7) cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis.
D R, Crawford   +5 more
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