Results 101 to 110 of about 77,694 (290)

Impact of Nanoparticle Stiffness on Endosomal Escape and Signaling Pathways in Cytosolic Delivery

Advanced Healthcare Materials, EarlyView.
Nanoparticle (NP) stiffness affects cellular uptake, but its impact on intracellular distribution remains unclear. This study synthesizes silica nanocapsules with varying stiffness, inspired by viral mechanisms, and applies assays to measure cellular uptake and escape efficiency.
Yali Zhang, Yue Hui, Zichao Guo, Dawei Liu, Yun Liu, Huajian Gao, Chun‐Xia Zhao   +6 more
wiley   +1 more source

METTL14‐Mediated M6A Modification of LINC01094 Induces Glucose Metabolic Reprogramming in Breast Cancer by Recruiting the PKM2/JMJD5 Complex

Advanced Science, EarlyView.
METTL14/IGF2BP2‐mediated m6A modification drives LINC01094 upregulation in BC. Then, LINC01094 interacts with PKM2 monomers to promote their dimerization, while serving as a flexible scaffold to facilitate the assembly of the PKM2/JMJD5 complex, synergistically stabilizing PKM2 dimers and enhancing their nuclear translocation.
Mengqi Wang, Zhaoxin Gao, Ruinan Zhao, Pan Zhou, Jingxi Chen, Hui Zhang, Yawen Wang, Wenjie Zhu, Peng Gao   +8 more
wiley   +1 more source

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

Biologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

In Situ Detection of Aspergillus 18S Ribosomal RNA in Invasive Pulmonary Apergillosis.

Internal Medicine, 1999
We attempted to evaluate the usefulness of in situ hybridization (ISH) in the specific diagnosis of Aspergillus pulmonary infection.We used an ISH technique using a multiple digoxigenin-incorporating probe, which was constructed by means of the polymerase chain reaction (PCR) from the 18S ribosomal RNA of Aspergillus fumigatus.We studied twelve ...
Yuji Ohtsuki, Eiji Hakoda, Takayuki Ikezoe, Hiroshi Sonobe, Hirokuni Taguchi, Makoto Kobayashi   +5 more
openaire   +4 more sources

Two Species of Canine Babesia in Australia: Detection and Characterization by PCR [PDF]

, 2003
The haemoprotozoan Babesia canis has been recognized in Australia for many years, and a second, smaller species has recently been discovered. Amplification and sequencing of a partial region of the 18S small subunit ribosomal RNA (rRNA) gene enabled ...
Irwin, P.J., Jefferies, R., Muhlnickel, C.J., Ryan, U.   +3 more
core   +2 more sources

Hypoglycemia Induces Diabetic Macrovascular Endothelial Dysfunction via Endothelial Cell PANoptosis, Macrophage Polarization, and VSMC Fibrosis

Advanced Science, EarlyView.
This study investigates hypoglycemia‐induced diabetic macrovascular endothelial dysfunction. It reveals that hypoglycemia triggers ZBP1‐dependent PANoptosis of endothelial cells, proinflammatory polarization of macrophages, and fibrosis of vascular smooth muscle cells (VSMCs) in diabetic mice.
Deyu Zuo, Yuce Peng, Guozhi Zhao, Zhesheng Cheng, Minghao Luo, Dingyi Lv, Shuting Chang, Na Li, Yanyao Huang, Xunjia Li, An He   +10 more
wiley   +1 more source

Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

BMC Plant Biology, 2010
Background Reference genes are commonly used as the endogenous normalisation measure for the relative quantification of target genes. The appropriate application of quantitative real-time PCR (RT-qPCR), however, requires the use of reference genes whose ...
Kundu Jiban K, Jarošová Jana
doaj   +1 more source

Validation of a PNA Clamping Method for Reducing Host DNA Amplification and Increasing Eukaryotic Diversity in Rhizosphere Microbiome Studies

Phytobiomes Journal, 2020
Protists and microscopic animals are important but poorly understood determinants of plant health. Plant-associated eukaryotes could be surveyed by high-throughput sequencing of 18S ribosomal RNA (rRNA) genes but the abundance of plant DNA in rhizosphere
Stephen J. Taerum, Blaire Steven, Daniel J. Gage, Lindsay R. Triplett   +3 more
doaj   +1 more source

Exposure to low pH induces molecular level changes in the marine worm, Platynereis dumerilii [PDF]

, 2015
© 2015 Elsevier Inc. Fossil fuel emissions and changes in net land use lead to an increase in atmospheric CO 2 concentration and a subsequent decrease of ocean pH. Noticeable effects on organisms' calcification rate, shell structure and energy metabolism
Hardege, Jörg D., Lerebours, Adelaide, Rotchell, Jeanette M., Wäge, Janine   +3 more
core   +1 more source

MYC Binding Near Transcriptional End Sites Regulates Basal Gene Expression, Read‐Through Transcription, and Intragenic Contacts

Advanced Science, EarlyView.
MYC is a transcription factor (TF) that binds DNA near transcriptional start sites (TSSs) and within enhancer elements. Here, unappreciated sites of MYC binding in the vicinity of transcriptional end sites (TESs) of many genes in multiple cell types in association with numerous other TFs are described previously.
Huabo Wang, Bingwei Ma, Taylor Stevens, Jessica Knapp, Jie Lu, Edward V. Prochownik   +5 more
wiley   +1 more source