Results 21 to 30 of about 587,009 (267)
Dynamics of ribosomes in mRNA translation under steady and non-steady state conditions [PDF]
Phys. Rev. E 101, 062404 (2020), 2020 Recent advances in DNA sequencing and fluorescence imaging have made it possible to monitor the dynamics of ribosomes actively engaged in messenger RNA (mRNA) translation. Here, we model these experiments within the inhomogeneous totally asymmetric simple exclusion process (TASEP) using realistic kinetic parameters.
arxiv +1 more source
Mediating Ribosomal Competition by Splitting Pools [PDF]
LCSS Vol 5 Issue 5 (Nov 2020), 2020 Synthetic biology constructs often rely upon the introduction of "circuit" genes into host cells, in order to express novel proteins and thus endow the host with a desired behavior. The expression of these new genes "consumes" existing resources in the cell, such as ATP, RNA polymerase, amino acids, and ribosomes. Ribosomal competition among strands of
arxiv +1 more source
Karyotypic analysis and isolation of four DNA markers of the scleractinian coral Favites pentagona (Esper, 1795) (Scleractinia, Anthozoa, Cnidaria) [PDF]
Comparative Cytogenetics, 2022 We performed conventional and molecular cytogenetic studies on the Favites pentagona Esper, 1795, a scleractinian coral mostly found along the west coast of Japan. Karyotype analysis of F.
Rei Kawakami +6 more
doaj +3 more sources
Recent Advances on the Structure and Function of RNA Acetyltransferase Kre33/NAT10
Cells, 2019 Ribosome biogenesis is one of the most energy demanding processes in the cell. In eukaryotes, the main steps of this process occur in the nucleolus and include pre-ribosomal RNA (pre-rRNA) processing, post-transcriptional modifications, and assembly of ...
Sophie Sleiman, Francois Dragon
doaj +1 more source
Evaluation of qPCR reference genes for taimen (Hucho taimen) under heat stress
Scientific Reports, 2022 As a powerful and attractive method for detecting gene expression, qRT-PCR has been broadly used in aquaculture research. Understanding the biology of taimen (Hucho taimen) has drawn increasing interest because of its ecological and economic value ...
Xiaoxing Yang +9 more
doaj +1 more source
Power series method for solving TASEP-based models of mRNA translation [PDF]
S Scott and J Szavits-Nossan 2020 Phys. Biol. 17 015004, 2020 We develop a method for solving mathematical models of messenger RNA (mRNA) translation based on the totally asymmetric simple exclusion process (TASEP). Our main goal is to demonstrate that the method is versatile and applicable to realistic models of translation.
arxiv +1 more source
Rcl1 Protein, a Novel Nuclease for 18 S Ribosomal RNA Production [PDF]
Journal of Biological Chemistry, 2011 In all forms of life, rRNAs for the small and large ribosomal subunit are co-transcribed as a single transcript. Although this ensures the equimolar production of rRNAs, it requires the endonucleolytic separation of pre-rRNAs to initiate rRNA production.
Darryl M. Horn +2 more
openaire +3 more sources
Use of Internal Controls to Increase Quantitative Capabilities of the Ribonuclease Protection Assay
BioTechniques, 1997 Through the use of two internal controls, we have developed an improved method of quantitating ribonuclease protection assay (RPA) results. A truncated sense RNA fragment and an antisense RNA fragment for the gene of interest were transcribed from PCR ...
Mary Jo J. Davis +2 more
doaj +1 more source
Stochastic theory of protein synthesis and polysome: ribosome profile on a single mRNA transcript [PDF]
Journal of Theoretical Biology, vol.289, 36-46 (2011), 2011 The process of polymerizing a protein by a ribosome, using a messenger RNA (mRNA) as the corresponding template, is called {\it translation}. Ribosome may be regarded as a molecular motor for which the mRNA template serves also as the track. Often several ribosomes may translate the same (mRNA) simultaneously.
arxiv +1 more source
Animals, 2021 We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp.
Dina M. Metwally +4 more
doaj +1 more source