Results 21 to 30 of about 37,099 (261)
Validation of suitable internal control genes for expression studies in aging. [PDF]
, 2009 Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA +9 more
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A parsimonay analysis of eukaryotic small subunit ribosomal RNA (″18 S″) sequences [PDF]
Archives Internationales de Physiologie, de Biochimie et de Biophysique, 1993 Using the principles of Felsenstein's DNA parsimony analysis program (package PHYLIP 3.2), a program with the capacity to handle data sets of the magnitude of ss rRNA has been developed. With this program we applied an heuristic approach to 83 s rRNA sequences from the Antwerp data bank, compared along 4230 positions of their alignment.
Arrotin, P., Demoulin, Vincent
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Use of Internal Controls to Increase Quantitative Capabilities of the Ribonuclease Protection Assay
BioTechniques, 1997 Through the use of two internal controls, we have developed an improved method of quantitating ribonuclease protection assay (RPA) results. A truncated sense RNA fragment and an antisense RNA fragment for the gene of interest were transcribed from PCR ...
Mary Jo J. Davis +2 more
doaj +1 more source
The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]
, 1992 The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert +3 more
core +1 more source
The terminal sequences of Bombyx mori 18S ribosomal RNA
Nucleic Acids Research, 1975 The 5' and 3' terminal T1 oligonucleotides of 32p-labelled B. mori 18S ribosomal RNA were isolated by a two dimensional electrophoretic (diagonal) technique. Nucleotide sequence analysis showed that the 3' terminal fragment, (G)AUCAUUAOH, is identical to that previously obtained from the 18S rRNA of several other eukaryotic species. The sequence of the
K U, Sprague, R A, Kramer, M B, Jackson
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Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]
, 2016 This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John +5 more
core +1 more source
Evaluation of qPCR reference genes for taimen (Hucho taimen) under heat stress
Scientific Reports, 2022 As a powerful and attractive method for detecting gene expression, qRT-PCR has been broadly used in aquaculture research. Understanding the biology of taimen (Hucho taimen) has drawn increasing interest because of its ecological and economic value ...
Xiaoxing Yang +9 more
doaj +1 more source
Rcl1 Protein, a Novel Nuclease for 18 S Ribosomal RNA Production [PDF]
Journal of Biological Chemistry, 2011 In all forms of life, rRNAs for the small and large ribosomal subunit are co-transcribed as a single transcript. Although this ensures the equimolar production of rRNAs, it requires the endonucleolytic separation of pre-rRNAs to initiate rRNA production.
Darryl M, Horn +2 more
openaire +2 more sources
PLoS ONE, 2015 Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is ...
Chunxiao Yang +3 more
doaj +1 more source
Animals, 2021 We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp.
Dina M. Metwally +4 more
doaj +1 more source