Results 21 to 30 of about 138,758 (334)
Stably expressed housekeeping genes across developmental stages in the two-spotted spider mite, Tetranychus urticae. [PDF]
PLoS ONE, 2015 Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory.
Chunxiao Yang +3 more
doaj +1 more source
RNA Biology, 2022 snR30/U17 is a highly conserved H/ACA RNA that is required for maturation of the small ribosomal subunit in eukaryotes. By base-pairing to the expansion segment 6 (ES6) of 18S ribosomal RNA (rRNA), the snR30 H/ACA Ribonucleoprotein (RNP) indirectly ...
Timothy J. Vos, Ute Kothe
doaj +1 more source
Primary structure of rabbit 18S ribosomal RNA determined by direct RNA sequence analysis [PDF]
Nucleic Acids Research, 1984 The primary structure of rabbit 18S ribosomal RNA was determined by nucleotide sequence analysis of the RNA directly. The rabbit rRNA was specifically cleaved with T1 ribonuclease, as well as with E. coli RNase H using a Pst 1 DNA linker to generate a specific set of overlapping fragments spanning the entire length of the molecule.
J F, Connaughton +3 more
openaire +2 more sources
Recent Advances on the Structure and Function of RNA Acetyltransferase Kre33/NAT10
Cells, 2019 Ribosome biogenesis is one of the most energy demanding processes in the cell. In eukaryotes, the main steps of this process occur in the nucleolus and include pre-ribosomal RNA (pre-rRNA) processing, post-transcriptional modifications, and assembly of ...
Sophie Sleiman, Francois Dragon
doaj +1 more source
18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA [PDF]
BioMed Research International, 2016 The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis.
Cory Ann Leonard +3 more
openaire +2 more sources
Frontiers in Veterinary Science, 2023 Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui +13 more
doaj +1 more source
Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]
, 2016 This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John +5 more
core +1 more source
A parsimonay analysis of eukaryotic small subunit ribosomal RNA (″18 S″) sequences [PDF]
Archives Internationales de Physiologie, de Biochimie et de Biophysique, 1993 Using the principles of Felsenstein's DNA parsimony analysis program (package PHYLIP 3.2), a program with the capacity to handle data sets of the magnitude of ss rRNA has been developed. With this program we applied an heuristic approach to 83 s rRNA sequences from the Antwerp data bank, compared along 4230 positions of their alignment.
Arrotin, P., Demoulin, Vincent
openaire +3 more sources
Nucleic Acids Research, 2012 16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth +6 more
semanticscholar +1 more source
Use of Internal Controls to Increase Quantitative Capabilities of the Ribonuclease Protection Assay
BioTechniques, 1997 Through the use of two internal controls, we have developed an improved method of quantitating ribonuclease protection assay (RPA) results. A truncated sense RNA fragment and an antisense RNA fragment for the gene of interest were transcribed from PCR ...
Mary Jo J. Davis +2 more
doaj +1 more source