PLoS ONE, 2015 Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is ...
Chunxiao Yang +3 more
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PLoS Genetics, 2022 Ribosomes are essential nanomachines responsible for protein production. Although ribosomes are present in every living cell, ribosome biogenesis dysfunction diseases, called ribosomopathies, impact particular tissues specifically.
Jonathan Delhermite +9 more
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Grass Snakes (Natrix natrix) as a Reservoir of Alaria alata and Other Parasites
Pathogens, 2022 The aim of the study was to investigate the occurrence of Alaria alata (Goeze, 1782) in fifty-one grass snakes (Natrix natrix) collected in Gostynińsko-Włocławski Landscape Park. Each snake was tested for the presence of A.
Aneta Bełcik +9 more
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Regulation of the cardiomyocyte transcriptome vs translatome by endothelin-1 and insulin: translational regulation of 5' terminal oligopyrimidine tract (TOP) mRNAs by insulin [PDF]
, 2010 Background: Changes in cellular phenotype result from underlying changes in mRNA transcription and translation. Endothelin-1 stimulates cardiomyocyte hypertrophy with associated changes in mRNA/protein expression and an increase in the rate of protein ...
Clerk, Angela +5 more
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Cleavage of adenovirus messenger RNA and of 28S and 18S ribosomal RNA by RNase III. [PDF]
Proceedings of the National Academy of Sciences, 1975 Escherichia coli ribonuclease III cleaves adenovirus messenger RNA and mammalian 28S and 18S ribosomal RNA. Fragmentation is not random, but in each case a specific collection of products is generated. This points to the potential use of the enzyme as a tool for specific fragmentation of RNA. Cleavage by RNase III abolishes the capability of adenovirus
Heiner Westphal, Robert J. Crouch
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Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua
Plant Protection Science, 2019 Eight commonly used candidate reference genes, 18S ribosomal RNA (rRNA) (18S), 28S rRNA (28S), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1α), ribosomal protein L7 (RPL7), Alpha-tubulin (α-TUB), and TATA ...
Junjie Liu +5 more
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A parsimonay analysis of eukaryotic small subunit ribosomal RNA (″18 S″) sequences [PDF]
Archives Internationales de Physiologie, de Biochimie et de Biophysique, 1993 Using the principles of Felsenstein's DNA parsimony analysis program (package PHYLIP 3.2), a program with the capacity to handle data sets of the magnitude of ss rRNA has been developed. With this program we applied an heuristic approach to 83 s rRNA sequences from the Antwerp data bank, compared along 4230 positions of their alignment.
Vincent Demoulin, Pascal Arrotin
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Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction [PDF]
, 2005 The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania ...
Barroso, Paola Andrea +11 more
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Evaluating RNA status for RT-PCR in extracts of postmortem human brain tissue
BioTechniques, 2004 Total RNA was extracted from 105 individual postmortem human brain samples representing a range of postmortem conditions. To improve upon parameters currently used to screen for RNA quality, electropherogram patterns generated by the Agilent Bioanalyzer ...
Christine L. Miller +4 more
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Ribosomal Dysfunction Is a Common Pathomechanism in Different Forms of Trichothiodystrophy
Cells, 2023 Mutations in a broad variety of genes can provoke the severe childhood disorder trichothiodystrophy (TTD) that is classified as a DNA repair disease or a transcription syndrome of RNA polymerase II.
Gaojie Zhu +8 more
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