Primary structure of rabbit 18S ribosomal RNA determined by direct RNA sequence analysis [PDF]
Nucleic Acids Research, 1984 The primary structure of rabbit 18S ribosomal RNA was determined by nucleotide sequence analysis of the RNA directly. The rabbit rRNA was specifically cleaved with T1 ribonuclease, as well as with E. coli RNase H using a Pst 1 DNA linker to generate a specific set of overlapping fragments spanning the entire length of the molecule.
John F. Connaughton +3 more
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Protecting the proteome: Eukaryotic cotranslational quality control pathways. [PDF]
, 2014 The correct decoding of messenger RNAs (mRNAs) into proteins is an essential cellular task. The translational process is monitored by several quality control (QC) mechanisms that recognize defective translation complexes in which ribosomes are stalled on
Bennett, Eric J, Lykke-Andersen, Jens
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Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids [PDF]
, 2012 © 2012 Matyášek et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and ...
Fulnecek, J +7 more
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Frontiers in Physiology, 2019 Hawthorn spider mite, Amphitetranychus viennensis Zacher, is one of the most devastating pests of deciduous fruit trees. The overall goal of this research is to develop a standardized protocol for real-time quantitative reverse transcription PCR (RT-qPCR)
Jing Yang +9 more
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An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme. [PDF]
PLoS Biology, 2013 A multitude of proteins and small nucleolar RNAs transiently associate with eukaryotic ribosomal RNAs to direct their modification and processing and the assembly of ribosomal proteins. Utp22 and Rrp7, two interacting proteins with no recognizable domain,
Jinzhong Lin +4 more
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Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing [PDF]
, 2002 Many analyses have examined subnucleolar structures in eukaryotic cells, but the relationship between morphological structures, pre-rRNA processing, and ribosomal particle assembly has remained unclear.
Gadal, O +6 more
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Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
Microbial Cell Factories, 2003 Background A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads.
Molin Søren +5 more
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Exploring valid reference genes for quantitative real-time PCR analysis in Sesamia inferens (Lepidoptera: Noctuidae). [PDF]
PLoS ONE, 2015 The pink stem borer, Sesamia inferens, which is endemic in China and other parts of Asia, is a major pest of rice and causes significant yield loss in this host plant. Very few studies have addressed gene expression in S. inferens. Quantitative real-time
Meng Sun +3 more
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40S ribosome biogenesis co-factors are essential for gametophyte and embryo development [PDF]
, 2013 Ribosome biogenesis is well described in Saccharomyces cerevisiae. In contrast only very little information is available on this pathway in plants. This study presents the characterization of five putative protein co-factors of ribosome biogenesis in ...
Bohnsack, Markus T. +6 more
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Evaluation of three molecular markers for identification of European primary parasitoids of cereal aphids and their hyperparasitoids. [PDF]
PLoS ONE, 2017 Aphids are major pests of cereal crops and a suite of hymenopteran primary parasitoids play an important role in regulating their populations. However, hyperparasitoids may disrupt the biocontrol services provided by primary parasitoids.
Zhengpei Ye +3 more
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