Mitochondrial DNA Variation in the Aging Human Cerebral Cortex and Cerebellum
Aging Cell, Volume 25, Issue 1, January 2026.We used cerebellum and frontal cortex whole genome sequencing data from the North American Brain Expression Consortium to investigate mitochondrial DNA deletions, single nucleotide variants, and copy number. We found significant differences between brain regions and age effects for all these metrics, which illustrate mitochondrial changes in healthy ...
Audrey A. Omidsalar +13 more
wiley +1 more source
Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
BMC Research Notes, 2018 Objective Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. Results Key modifications
Katelyn M. Kimble +2 more
doaj +1 more source
Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
FEBS Letters, Volume 599, Issue 24, Page 3669-3684, December 2025.The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji +3 more
wiley +1 more source
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Nucleic Acids Research, 2007 The publication of a complete genome sequence is usually accompanied by annotations of its genes. In contrast to protein coding genes, genes for ribosomal RNA (rRNA) are often poorly or inconsistently annotated.
K. Lagesen +5 more
semanticscholar +1 more source
Molecular Identification of the Nematode Eustrongylides ignotus in Mandarin Fish, Siniperca chuatsi
Aquaculture, Fish and Fisheries, Volume 5, Issue 6, December 2025.ABSTRACT Mandarin fish (Siniperca chuatsi) is a high‐value economic freshwater fish in China; however, the frequent occurrence of diseases threatens its production. To this end, we explored the impact of a nematode parasite newly found in the abdomen of mandarin fish collected from Pudong, Shanghai, China.
Jin‐hua Gao +8 more
wiley +1 more source
BMC Plant Biology, 2010 Background In flowering plants and animals the most common ribosomal RNA genes (rDNA) organisation is that in which 35S (encoding 18S-5.8S-26S rRNA) and 5S genes are physically separated occupying different chromosomal loci.
Garcia Sònia +3 more
doaj +1 more source
Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses [PDF]
, 2017 Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to the high concentration of ...
Acevedo, Raúl Maximiliano +4 more
core +1 more source
Operon conservation and the evolution of trans-splicing in the phylum Nematoda [PDF]
, 2006 The nematode Caenorhabditis elegans is unique among model animals in that many of its genes are cotranscribed as polycistronic pre-mRNAs from operons.
Bektesh +53 more
core +3 more sources
Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
Caryologia, 2021 Ribosomal DNAs and various banding patterns are landmarks in molecular cytogenetics providing useful information for karyotyping and addressing individual chromosomes.
Ahmet L. Tek +3 more
doaj
Chromosomal diversification and karyotype evolution of diploids in the cytologically diverse genus Prospero (Hyacinthaceae) [PDF]
, 2013 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work ...
Andrew R Leitch +7 more
core +2 more sources