Results 51 to 60 of about 538,024 (260)

Charging State Analysis of Transfer RNA from an α-proteobacterium

Bio-Protocol, 2020
Transfer RNA (tRNA) is an essential link between the genetic code and proteins. During the process of translation, tRNA is charged with its cognate amino acid and delivers it to the ribosome, thus serving as a substrate of protein synthesis.
Liang Yin, Caroline Harwood
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Complete chloroplast genome sequence of Chrysosplenium ramosum and Chrysosplenium alternifolium (Saxifragaceae)

Mitochondrial DNA. Part B. Resources, 2020
The complete chloroplast genome of Chrysosplenium ramosum Maxim. and Chrysosplenium alternifolium L. were reported in this study. The chloroplast genomes were 153,460 bp for C. ramosum and 152,619 bp for C. alternifolium.
Wen-jie Yan, Hong Liu, Tian-ge Yang, Rui Liao, Rui Qin   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Analysis of tRNA halves (tsRNAs) in serum from cattle challenged with bovine viral diarrhea virus

Genetics and Molecular Biology
Acute infections of bovine viral diarrhea virus (BVDV) lead to a range of clinical presentations. Laboratory tests for detection depend on collection of samples during a short viremia. Acutely infected animals remain largely undiagnosed.
Tasia M. Taxis, Fernando V. Bauermann, Julia F. Ridpath, Eduardo Casas   +3 more
doaj   +1 more source

Comparative analysis of RNA families reveals distinct repertoires for each domain of life.

PLoS Computational Biology, 2012
The RNA world hypothesis, that RNA genomes and catalysts preceded DNA genomes and genetically-encoded protein catalysts, has been central to models for the early evolution of life on Earth.
Marc P Hoeppner, Paul P Gardner, Anthony M Poole   +2 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

N2-methylguanosine and N2, N2-dimethylguanosine in cytosolic and mitochondrial tRNAs

Frontiers in RNA Research
Decoration of cellular RNAs with modified RNA nucleosides is an important layer of gene expression regulation. Throughout the transcriptome, RNA modifications influence the folding, stability and function of RNAs as well as their interactions with RNA ...
Jonny Petrosyan, Katherine E. Bohnsack
doaj   +1 more source

T7 RNA polymerase‐driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis

Microbial Biotechnology, 2017
Summary The majority of the good DNA editing techniques have been developed in Escherichia coli; however, Bacillus subtilis is better host for a plethora of synthetic biology and biotechnology applications. Reliable and efficient systems for the transfer
Mario Juhas, James W. Ajioka
doaj   +1 more source