Results 101 to 110 of about 915,194 (208)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Stage‐Specific RNA Turnover Drives Small RNA Dynamics in Arabidopsis–Colletotrichum Interactions

Plant Direct
Small RNAs (sRNAs) are key regulators of plant defense and have been implicated in cross‐kingdom interactions with pathogens. The hemibiotrophic fungus Colletotrichum higginsianum infects Arabidopsis thaliana through three stages: appressorial ...
Carolina E. Armijos, Thi‐Thu‐Huyen Chu, Richard J. O' Connell, Blake C. Meyers, Patricia Baldrich   +4 more
doaj   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Circular versus linear RNA topology: different modes of RNA-RNA interactions in vitro and in human cells. [PDF]

RNA Biol, 2021
Petkovic S, Graff S, Feller N, Berghaus J, Ruppert VP, Dülfer J, Sczakiel G.   +6 more
europepmc   +1 more source

Systematic identification of variant-specific RNA structure-small molecule interactions exemplified by RNA G-quadruplexes

Nature Communications
Individual genetic variations, such as cancer-associated somatic mutations, alter RNA structures, thereby potentially enhancing or inhibiting the binding of RNA-targeting small molecules. However, to date, no approach has been available to identify these
Emi Miyashita, Kazumitsu Onizuka, Yutong Chen, Hiroki Yoshida, Hina Hatayama, Shunya Ishikawa, Peijie Yan, Takahito Hasegawa, Mamiko Ozawa, Kaho Maeta, Fumi Nagatsugi, Hirohide Saito, Kaoru R. Komatsu   +12 more
doaj   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

In vivo reconstitution finds multivalent RNA-RNA interactions as drivers of mesh-like condensates. [PDF]

Elife, 2021
Ma W, Zhen G, Xie W, Mayr C.
europepmc   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Contribution of RNA-RNA Interactions Mediated by the Genome Packaging Signals for the Selective Genome Packaging of Influenza A Virus. [PDF]

J Virol, 2022
Miyamoto S, Muramoto Y, Shindo K, Fujita-Fujiharu Y, Morikawa T, Tamura R, Gilmore JL, Nakano M, Noda T.   +8 more
europepmc   +1 more source