Arabidopsis C-terminal domain phosphatase-like 1 functions in miRNA accumulation and DNA methylation. [PDF]
Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated
In Sil Jeong+7 more
doaj +1 more source
Morphological and Molecular Characterization of Pseudocercospora chionanthi-retusi Causing Leaf Spot on Chionanthus retusus in Korea [PDF]
Leaves of Chionanthus retusus were found to be damaged by leaf spot disease associated with a fungus in Iksan, Korea. Leaf spots were angular to irregular, vein-limited, scattered, 1–8 mm diameter, brownish-gray to dark brown when dry, with heavy ...
In-Young Choi+3 more
doaj +1 more source
Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis. [PDF]
The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression.
Chory, Joanne+9 more
core +2 more sources
Heterochromatic siRNAs and DDM1 independently silence aberrant 5S rDNA transcripts in Arabidopsis. [PDF]
5S ribosomal RNA gene repeats are arranged in heterochromatic arrays (5S rDNA) situated near the centromeres of Arabidopsis chromosomes. The chromatin remodeling factor DDM1 is known to maintain 5S rDNA methylation patterns while silencing transcription ...
Todd Blevins+3 more
doaj +1 more source
Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage [PDF]
The p53 tumour suppressor regulates the transcription initiation of selected genes by binding to specific DNA sequences at their promoters. Here we report a novel role of p53 in transcription elongation in human cells.
Boros, I.M. (Imre M.)+7 more
core +2 more sources
Transcription in vitro of ø29 DNA and EcoRI fragments by Bacillus subtilis RNA polymerase [PDF]
EcoRI fragments A, B and C produced from linear φ29 DNA, but not D or E fragments, are transcribed by purified Bacillus subtilis RNA polymerase. The transcription of fragments A and C is initiated preferentially with GTP and to a lesser extent with ATP ...
Inciarte, M. R.+2 more
core +1 more source
The trans-activation domain of the sporulation response regulator Spo0A revealed by X-ray crystallography [PDF]
Sporulation in Bacillus involves the induction of scores of genes in a temporally and spatially co-ordinated programme of cell development. Its initiation is under the control of an expanded two-component signal transduction system termed a phosphorelay.
Baldus J.M.+32 more
core +1 more source
In vitro transcription of the avian retrovirus genome by the alpha form of the viral RNA-directed DNA polymerase [PDF]
The nature of transcription of the avian retrovirus RNA genome by the alpha form of the viral RNA-directed DNA polymerase has been investigated. Transcription was most efficient when Mn2+ was provided as the divalent metal ion. The patterns of DNA transcription using 70S RNA, 35S RNA-tRNAtrp, or 35S RNA-oligo(dT)12-18 template-primer complexes by the ...
Marc S. Collett+2 more
openalex +4 more sources
HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors [PDF]
RNA and DNA aptamers specific for HIV-1 reverse transcriptase (RT) can inhibit reverse transcription in vitro. RNA aptamers have been shown to potently block HIV-1 replication in culture.
Fisher, Timothy S+2 more
core +3 more sources
Pol IV-Dependent siRNA Production is Reduced in Brassica rapa
Plants produce a diverse array of small RNA molecules capable of gene regulation, including Pol IV-dependent short interfering (p4-si)RNAs that trigger transcriptional gene silencing.
Rebecca A. Mosher+2 more
doaj +1 more source