Results 1 to 10 of about 167,761 (271)
RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA [PDF]
Open Biology, 2015 MicroRNAs (miRNAs) integrate with Argonaut (Ago) to create the RNA-induced silencing complex, and regulate gene expression by silencing target mRNAs. RNA editing of miRNA may affect miRNA processing, assembly of the Ago complex and target mRNA binding ...
Yalei Cui +2 more
doaj +6 more sources
Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence [PDF]
eLife, 2020 The exchange of small RNAs (sRNAs) between hosts and pathogens can lead to gene silencing in the recipient organism, a mechanism termed cross-kingdom RNAi (ck-RNAi).
Florian Dunker +10 more
doaj +7 more sources
The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine* [PDF]
Journal of Biological Chemistry, 2009 RNA interference is a powerful mechanism of gene silencing that underlies many aspects of eukaryotic biology. On the molecular level, RNA interference is mediated by a family of ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs ...
Ashley J. Pratt, I. MacRae
semanticscholar +6 more sources
Comprehensive Evolutionary Analysis of the Major RNA-Induced Silencing Complex Members [PDF]
Scientific Reports, 2018 RNA-induced silencing complex (RISC) plays a critical role in small interfering RNA (siRNA) and microRNAs (miRNA) pathways. Accumulating evidence has demonstrated that the major RISC members (AGO, DICER, TRBP, PACT and GW182) represent expression ...
Rui Zhang +8 more
semanticscholar +4 more sources
Molecular Requirements for RNA-induced Silencing Complex Assembly in the Drosophila RNA Interference Pathway* [PDF]
Journal of Biological Chemistry, 2005 Complexes in the Drosophila RNA-induced silencing complex (RISC) assembly pathway can be resolved using native gel electrophoresis, revealing an initiator called R1, an intermediate called R2, and an effector called R3 (now referred to as holo-RISC ...
John W Pham, E. Sontheimer
semanticscholar +6 more sources
Increased RNA‐induced silencing complex (RISC) activity contributes to hepatocellular carcinoma [PDF]
Hepatology, 2011 There is virtually no effective treatment for advanced hepatocellular carcinoma (HCC) and novel targets need to be identified to develop effective treatment.
B. Yoo +8 more
semanticscholar +6 more sources
The RNA-Induced Silencing Complex Is a Mg2+-Dependent Endonuclease [PDF]
Current Biology, 2004 In the Drosophila and mammalian RNA interference (RNAi) pathways, target RNA destruction is catalyzed by the siRNA-guided, RNA-induced silencing complex (RISC). RISC has been proposed to be an siRNA-directed endonuclease, catalyzing cleavage of a single phosphodiester bond on the RNA target.
D. Schwarz, Yukihide Tomari, P. Zamore
semanticscholar +6 more sources
STAR Protocols, 2022 Summary: Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation
Yajie Gao +3 more
doaj +4 more sources
Biochemical mechanisms of the RNA-induced silencing complex [PDF]
Cell Research, 2007 In less than 10 years since its inception, RNA interference (RNAi) has had extraordinary impact on biomedical science. RNAi has been demonstrated to influence numerous biological and disease pathways. Development and adoption of RNAi technologies have been prolific ranging from basic loss-of-function tools, genome-wide screening libraries to ...
Z. Paroo, Qinghua Liu, Xiaodong Wang
semanticscholar +5 more sources
PLoS ONE, 2010 Background GW/P bodies are cytoplasmic ribonucleoprotein-rich foci involved in microRNA (miRNA)-mediated messenger RNA (mRNA) silencing and degradation. The mRNA regulatory functions within GW/P bodies are mediated by GW182 and its binding partner hAgo2 ...
J. J. Moser, M. Fritzler
semanticscholar +7 more sources