RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA [PDF]
Open Biology, 2015 MicroRNAs (miRNAs) integrate with Argonaut (Ago) to create the RNA-induced silencing complex, and regulate gene expression by silencing target mRNAs. RNA editing of miRNA may affect miRNA processing, assembly of the Ago complex and target mRNA binding ...
Yalei Cui +2 more
doaj +7 more sources
Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence [PDF]
eLife, 2020 The exchange of small RNAs (sRNAs) between hosts and pathogens can lead to gene silencing in the recipient organism, a mechanism termed cross-kingdom RNAi (ck-RNAi).
Florian Dunker +10 more
doaj +8 more sources
Comprehensive Evolutionary Analysis of the Major RNA-Induced Silencing Complex Members. [PDF]
Sci Rep, 2018 RNA-induced silencing complex (RISC) plays a critical role in small interfering RNA (siRNA) and microRNAs (miRNA) pathways. Accumulating evidence has demonstrated that the major RISC members (AGO, DICER, TRBP, PACT and GW182) represent expression ...
Zhang R +8 more
europepmc +6 more sources
Critical role of backbone coordination in the mRNA recognition by RNA induced silencing complex [PDF]
Communications Biology, 2021 Lizhe Zhu et al. use a quasi Markov State Model built from extensive molecular dynamics simulations to elucidate the dynamics of RISC-mRNA interactions. Their results provide further insight on how a target nucleotide is positioned before recognition by ...
Lizhe Zhu +5 more
doaj +7 more sources
Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells [PDF]
STAR Protocols, 2022 Summary: Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation
Yajie Gao +3 more
doaj +5 more sources
The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine [PDF]
Journal of Biological Chemistry, 2009 RNA interference is a powerful mechanism of gene silencing that underlies many aspects of eukaryotic biology. On the molecular level, RNA interference is mediated by a family of ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs ...
Ashley J. Pratt, Ian J. MacRae
semanticscholar +5 more sources
Ribosomal proteins L5 and L11 co-operatively inactivate c-Myc via RNA-induced silencing complex [PDF]
Oncogene, 2013 Oncogene MYC is highly expressed in many human cancers and functions as a global regulator of ribosome biogenesis. Previously, we reported that ribosomal protein (RP) L11 binds to c-Myc and inhibits its transcriptional activity in response to ribosomal ...
Jingling Liao +3 more
semanticscholar +4 more sources
Retraction: ‘RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA’ (2015), by Cui et al. [PDF]
Open Biology, 2023 Open Biology editorial team.
doaj +3 more sources
Journal of Neuroscience, 2015 MicroRNAs (miRNAs) associate with Argonaute (Ago), GW182, and FXR1 proteins to form RNA-induced silencing complexes (RISCs). RISCs represent a critical checkpoint in the regulation and bioavailability of miRNAs. Recent studies have revealed dysregulation
Przemysław Lewkowicz +7 more
openalex +3 more sources
Structure of Aquifex aeolicus Argonaute Highlights Conformational Flexibility of the PAZ Domain as a Potential Regulator of RNA-induced Silencing Complex Function [PDF]
Journal of Biological Chemistry, 2006 Gene silencing mediated by RNA interference requires the sequence-specific recognition of target mRNA by the endonuclease Argonaute, the primary enzymatic component of the RNA-induced silencing complex. We report the crystal structure of Aquifex aeolicus
Umar Rashid +5 more
openalex +2 more sources