RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA [PDF]
Open Biology, 2015 MicroRNAs (miRNAs) integrate with Argonaut (Ago) to create the RNA-induced silencing complex, and regulate gene expression by silencing target mRNAs. RNA editing of miRNA may affect miRNA processing, assembly of the Ago complex and target mRNA binding ...
Yalei Cui +2 more
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Critical role of backbone coordination in the mRNA recognition by RNA induced silencing complex [PDF]
Communications Biology, 2021 Lizhe Zhu et al. use a quasi Markov State Model built from extensive molecular dynamics simulations to elucidate the dynamics of RISC-mRNA interactions. Their results provide further insight on how a target nucleotide is positioned before recognition by ...
Lizhe Zhu +5 more
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Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence [PDF]
eLife, 2020 The exchange of small RNAs (sRNAs) between hosts and pathogens can lead to gene silencing in the recipient organism, a mechanism termed cross-kingdom RNAi (ck-RNAi).
Florian Dunker +10 more
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Comprehensive Evolutionary Analysis of the Major RNA-Induced Silencing Complex Members. [PDF]
Sci Rep, 2018 AbstractRNA-induced silencing complex (RISC) plays a critical role in small interfering RNA (siRNA) and microRNAs (miRNA) pathways. Accumulating evidence has demonstrated that the major RISC members (AGO, DICER, TRBP, PACT and GW182) represent expression discrepancies or multiple orthologues/paralogues in different species.
Zhang R +8 more
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Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells. [PDF]
STAR Protoc, 2022 Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation of RNA probes, the target RNA, and Drosophila cell lysates for RISC assembly assay.
Gao Y, Zhu Y, Sun Q, Chen D.
europepmc +5 more sources
Is circulating exosome carry Staphylococcal nuclease domain-containing protein 1, a component of RNA-induced silencing complex? [PDF]
Genes Dis, 2021 Shiragannavar VD +2 more
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The RNA-Induced Silencing Complex Is a Mg2+-Dependent Endonuclease [PDF]
Current Biology, 2004 In the Drosophila and mammalian RNA interference (RNAi) pathways, target RNA destruction is catalyzed by the siRNA-guided, RNA-induced silencing complex (RISC). RISC has been proposed to be an siRNA-directed endonuclease, catalyzing cleavage of a single phosphodiester bond on the RNA target.
Dianne S. Schwarz +2 more
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Biochemical mechanisms of the RNA-induced silencing complex [PDF]
Cell Research, 2007 In less than 10 years since its inception, RNA interference (RNAi) has had extraordinary impact on biomedical science. RNAi has been demonstrated to influence numerous biological and disease pathways. Development and adoption of RNAi technologies have been prolific ranging from basic loss-of-function tools, genome-wide screening libraries to ...
Zain Paroo, Qinghua Liu, Xiaodong Wang
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Ribosomal proteins L5 and L11 co-operatively inactivate c-Myc via RNA-induced silencing complex. [PDF]
Oncogene, 2014 Oncogene MYC is highly expressed in many human cancers and functions as a global regulator of ribosome biogenesis. Previously, we reported that ribosomal protein (RP) L11 binds to c-Myc and inhibits its transcriptional activity in response to ribosomal stress. Here, we show that RPL5, co-operatively with RPL11, guides the RNA-induced silencing complex (
Liao JM, Zhou X, Gatignol A, Lu H.
europepmc +6 more sources
The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine [PDF]
Journal of Biological Chemistry, 2009 RNA interference is a powerful mechanism of gene silencing that underlies many aspects of eukaryotic biology. On the molecular level, RNA interference is mediated by a family of ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs), which can be programmed to target virtually any nucleic acid sequence for silencing.
Ashley J. Pratt, Ian J. MacRae
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