Increased RNA-induced silencing complex (RISC) activity contributes to hepatocellular carcinoma [PDF]
Hepatology, 2011 There is virtually no effective treatment for advanced hepatocellular carcinoma (HCC) and novel targets need to be identified to develop effective treatment.
Byoung Kwon Yoo +8 more
semanticscholar +5 more sources
The RNA-Induced Silencing Complex Is a Mg2+-Dependent Endonuclease [PDF]
Current Biology, 2004 In the Drosophila and mammalian RNA interference (RNAi) pathways, target RNA destruction is catalyzed by the siRNA-guided, RNA-induced silencing complex (RISC). RISC has been proposed to be an siRNA-directed endonuclease, catalyzing cleavage of a single phosphodiester bond on the RNA target.
Dianne S. Schwarz +2 more
semanticscholar +4 more sources
Telomerase, an Accomplice of RNA-induced Silencing Complex in Hepatocellular Carcinoma [PDF]
Gene Expression, 2023 Lakshana D. Puttahanumantharayappa +4 more
semanticscholar +3 more sources
The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts [PDF]
Genes & Development, 2016 In this study, Shimada et al. use fission yeast to investigate how small RNA-loaded Argonaute protein complexes target chromatin to mediate silencing and show that transcription of the target locus is essential for RNA-directed formation of ...
Yukiko Shimada, Fabio Mohn, Marc Bühler
semanticscholar +4 more sources
A 5′-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation [PDF]
Silence, 2011 BackgroundMicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).ResultsOur analyses of fly, worm and mouse miRNA sequence data reveal that the 5′-U is recognized after miRNA production.
Hervé Seitz +2 more
semanticscholar +6 more sources
Molecular Requirements for RNA-induced Silencing Complex Assembly in the Drosophila RNA Interference Pathway [PDF]
Journal of Biological Chemistry, 2005 Complexes in the Drosophila RNA-induced silencing complex (RISC) assembly pathway can be resolved using native gel electrophoresis, revealing an initiator called R1, an intermediate called R2, and an effector called R3 (now referred to as holo-RISC ...
John Pham, Erik J. Sontheimer
semanticscholar +4 more sources
RNA-induced initiation of transcriptional silencing (RITS) complex structure and function [PDF]
RNA Biology, 2019 Heterochromatic regions of the genome are epigenetically regulated to maintain a heritable ‘“silent state”’. In fission yeast and other organisms, epigenetic silencing is guided by nascent transcripts, which are targeted by the RNA interference pathway ...
Sonali Bhattacharjee +2 more
semanticscholar +4 more sources
Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach [PDF]
Proceedings of the National Academy of Sciences, 2012 Identifying targets is critical for understanding the biological effects of microRNA (miRNA) expression. The challenge lies in characterizing the cohort of targets for a specific miRNA, especially when targets are being actively down-regulated in miRNA– RNA-induced silencing complex (RISC)–messengerRNA (mRNA) complexes.
Xiaolu A. Cambronne +3 more
semanticscholar +4 more sources
Biochemical mechanisms of the RNA-induced silencing complex [PDF]
Cell Research, 2007 In less than 10 years since its inception, RNA interference (RNAi) has had extraordinary impact on biomedical science. RNAi has been demonstrated to influence numerous biological and disease pathways. Development and adoption of RNAi technologies have been prolific ranging from basic loss-of-function tools, genome-wide screening libraries to ...
Zain Paroo, Qinghua Liu, Xiaodong Wang
semanticscholar +4 more sources
Synaptotagmin 11 interacts with components of the RNA‐induced silencing complex RISC in clonal pancreatic β‐cells [PDF]
FEBS Letters, 2014 Synaptotagmins are two C2 domain‐containing transmembrane proteins. The function of calcium‐sensitive members in the regulation of post‐Golgi traffic has been well established whereas little is known about the calcium‐insensitive isoforms constituting half of the protein family. Novel binding partners of synaptotagmin 11 were identified in β‐cells.
Alexandra Milochau +11 more
semanticscholar +4 more sources