RNA-induced initiation of transcriptional silencing (RITS) complex structure and function [PDF]
RNA Biology, 2019 Heterochromatic regions of the genome are epigenetically regulated to maintain a heritable ‘“silent state”’. In fission yeast and other organisms, epigenetic silencing is guided by nascent transcripts, which are targeted by the RNA interference pathway ...
S. Bhattacharjee +2 more
semanticscholar +5 more sources
A 5′-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation [PDF]
Silence, 2011 BackgroundMicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).ResultsOur analyses of fly, worm and mouse miRNA sequence data reveal that the 5′-U is recognized after miRNA production.
H. Seitz, J. S. Tushir, P. Zamore
semanticscholar +7 more sources
Ribosomal Proteins L5 and L11 Cooperatively Inactivate c-Myc via RNA-induced Silencing Complex [PDF]
Oncogene, 2013 Oncogene MYC is highly expressed in many human cancers and functions as a global regulator of ribosome biogenesis. Previously, we reported that ribosomal protein (RP) L11 binds to c-Myc and inhibits its transcriptional activity in response to ribosomal ...
Jun-Ming Liao +3 more
semanticscholar +5 more sources
The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts [PDF]
Genes & Development, 2016 In this study, Shimada et al. use fission yeast to investigate how small RNA-loaded Argonaute protein complexes target chromatin to mediate silencing and show that transcription of the target locus is essential for RNA-directed formation of ...
Y. Shimada, Fabio Mohn, M. Bühler
semanticscholar +5 more sources
Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach [PDF]
Proceedings of the National Academy of Sciences, 2012 Identifying targets is critical for understanding the biological effects of microRNA (miRNA) expression. The challenge lies in characterizing the cohort of targets for a specific miRNA, especially when targets are being actively down-regulated in miRNA– RNA-induced silencing complex (RISC)–messengerRNA (mRNA) complexes.
Xiaolu A. Cambronne +3 more
semanticscholar +5 more sources
Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity. [PDF]
Proceedings of the National Academy of Sciences, 2004 RNA interference is carried out by the small double-stranded RNA-induced silencing complex (RISC). The RISC-bound small RNA guides the RISC complex to identify and cleave mRNAs with complementary sequences. The proteins that make up the RISC complex and cleave mRNA have not been unequivocally defined.
T. A. Rand +3 more
semanticscholar +5 more sources
Critical role of backbone coordination in the mRNA recognition by RNA induced silencing complex [PDF]
Communications Biology, 2021 Lizhe Zhu et al. use a quasi Markov State Model built from extensive molecular dynamics simulations to elucidate the dynamics of RISC-mRNA interactions. Their results provide further insight on how a target nucleotide is positioned before recognition by ...
Lizhe Zhu +5 more
doaj +4 more sources
RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators [PDF]
Proceedings of the National Academy of Sciences, 2013 The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including protein kinase RNA activator (PACT), transactivation response RNA binding protein (TRBP), and Dicer, that process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation.
A. Redfern +20 more
semanticscholar +5 more sources
Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody. [PDF]
Journal of Immunological Methods, 2006 MicroRNAs (miRNAs) are short RNA molecules responsible for post-transcriptional gene silencing by the degradation or translational inhibition of their target messenger RNAs (mRNAs). This process of gene silencing, known as RNA interference (RNAi), is mediated by highly conserved Argonaute (Ago) proteins which are the key components of the RNA induced ...
K. Ikeda +5 more
semanticscholar +7 more sources
Dysregulated RNA-Induced Silencing Complex (RISC) Assembly within CNS Corresponds with Abnormal miRNA Expression during Autoimmune Demyelination [PDF]
Journal of Neuroscience, 2015 MicroRNAs (miRNAs) associate with Argonaute (Ago), GW182, and FXR1 proteins to form RNA-induced silencing complexes (RISCs). RISCs represent a critical checkpoint in the regulation and bioavailability of miRNAs. Recent studies have revealed dysregulation
P. Lewkowicz +7 more
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