Molecular Requirements for RNA-induced Silencing Complex Assembly in the Drosophila RNA Interference Pathway [PDF]
Journal of Biological Chemistry, 2005 Complexes in the Drosophila RNA-induced silencing complex (RISC) assembly pathway can be resolved using native gel electrophoresis, revealing an initiator called R1, an intermediate called R2, and an effector called R3 (now referred to as holo-RISC).
John Pham, Erik J. Sontheimer
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Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach [PDF]
Proceedings of the National Academy of Sciences, 2012 Identifying targets is critical for understanding the biological effects of microRNA (miRNA) expression. The challenge lies in characterizing the cohort of targets for a specific miRNA, especially when targets are being actively down-regulated in miRNA– RNA-induced silencing complex (RISC)–messengerRNA (mRNA) complexes.
Xiaolu A. Cambronne +3 more
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miR-7 is recruited to the high molecular weight RNA-induced silencing complex in CD8+ T cells upon activation and suppresses IL-2 signaling. [PDF]
RNA, 2023 Toivakka M +6 more
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Classification of and detection techniques for RNAi-induced effects in GM plants [PDF]
Frontiers in Plant ScienceRNA interference (RNAi) is a biotechnological tool used for gene silencing in plants, with both endogenous and exogenous applications. Endogenous approaches, such as host-induced gene silencing (HIGS), involve genetically modified (GM) plants, while ...
Cecilia Diaz +6 more
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In Arabidopsis thaliana, RNA-Induced Silencing Complex-Loading of MicroRNAs Plays a Minor Regulatory Role During Photomorphogenesis Except for miR163. [PDF]
Front Plant Sci, 2022 Lakatos L, Groma G, Silhavy D, Nagy F.
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RNA-induced initiation of transcriptional silencing (RITS) complex structure and function [PDF]
RNA Biology, 2019 Heterochromatic regions of the genome are epigenetically regulated to maintain a heritable '"silent state"'. In fission yeast and other organisms, epigenetic silencing is guided by nascent transcripts, which are targeted by the RNA interference pathway. The key effector complex of the RNA interference pathway consists of small interfering RNA molecules
Sonali Bhattacharjee +2 more
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In Vitro Assembly of Plant RNA-Induced Silencing Complexes Facilitated by Molecular Chaperone HSP90 [PDF]
Molecular Cell, 2010 RNA-induced silencing complexes (RISCs) play central roles in posttranscriptional gene silencing. In plants, the mechanism of RISC assembly has remained elusive due to the lack of cell-free systems that recapitulate the process. In this report, we demonstrate that plant AGO1 protein synthesized by in vitro translation using an extract of evacuolated ...
Taichiro Iki +7 more
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Expression of concern: 'RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA' (2015), by Cui Y et al. [PDF]
Open Biol, 2023 Open Biology editorial team.
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Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity [PDF]
Proceedings of the National Academy of Sciences, 2004 RNA interference is carried out by the small double-stranded RNA-induced silencing complex (RISC). The RISC-bound small RNA guides the RISC complex to identify and cleave mRNAs with complementary sequences. The proteins that make up the RISC complex and cleave mRNA have not been unequivocally defined.
Tim Rand +3 more
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Retraction: ‘RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA’ (2015), by Cui et al. [PDF]
Open Biology, 2023 Open Biology editorial team.
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