The origin and effect of small RNA signaling in plants [PDF]
Frontiers in Plant Science, 2012 Given their sessile condition, land plants need to integrate environmental cues rapidly and send signal throughout the organism to modify their metabolism accordingly. Small RNA (sRNA) molecules are among the messengers that plant cells use to carry such
Jean-Sébastien eParent +2 more
doaj +3 more sources
In Vitro Assembly of Plant RNA-Induced Silencing Complexes Facilitated by Molecular Chaperone HSP90 [PDF]
Molecular Cell, 2010 RNA-induced silencing complexes (RISCs) play central roles in posttranscriptional gene silencing. In plants, the mechanism of RISC assembly has remained elusive due to the lack of cell-free systems that recapitulate the process. In this report, we demonstrate that plant AGO1 protein synthesized by in vitro translation using an extract of evacuolated ...
Taichiro Iki +7 more
openaire +5 more sources
RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
Molecular Therapy: Nucleic Acids, 2014 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Simon J Allison, Jo Milner
doaj +8 more sources
The RNA-binding Protein TDP-43 Selectively Disrupts MicroRNA-1/206 Incorporation into the RNA-induced Silencing Complex*♦ [PDF]
Journal of Biological Chemistry, 2014 Background: Regulation of microRNA activity independent of processing and biogenesis has not been demonstrated. Results: The RNA-binding protein, TDP-43, interacts with mature miR-1/miR-206, limiting their RNA-induced silencing complex (RISC) association
I. King +9 more
semanticscholar +2 more sources
Structure of Aquifex aeolicus Argonaute Highlights Conformational Flexibility of the PAZ Domain as a Potential Regulator of RNA-induced Silencing Complex Function* [PDF]
Journal of Biological Chemistry, 2007 Gene silencing mediated by RNA interference requires the sequence-specific recognition of target mRNA by the endonuclease Argonaute, the primary enzymatic component of the RNA-induced silencing complex. We report the crystal structure of Aquifex aeolicus
U. Rashid +5 more
semanticscholar +2 more sources
MicroRNAs are tightly associated with RNA-induced gene silencing complexes in vivo
Biochemical and Biophysical Research Communications, 2008 Previous work has shown that synthesized siRNA/miRNA is tightly associated with RNA-induced Gene Silencing Complexes (RISCs) in vitro. However, it is unknown if the endogenous miRNAs are also stably bound to RISC complexes in vivo in cells under physiological conditions.
M. Azim Surani +6 more
openaire +5 more sources
Life of RISC: Formation, action, and degradation of RNA-induced silencing complex.
Molecular Cell, 2021 Small RNAs regulate a wide variety of biological processes by repressing the expression of target genes at the transcriptional and post-transcriptional levels. To achieve these functions, small RNAs form RNA-induced silencing complex (RISC) together with a member of the Argonaute (AGO) protein family.
Hirotaka Iwakawa, Yukihide Tomari
semanticscholar +3 more sources
LINE-1 ORF1 Protein Localizes in Stress Granules with Other RNA-Binding Proteins, Including Components of RNA Interference RNA-Induced Silencing Complex [PDF]
Molecular and Cellular Biology, 2007 J. Goodier +3 more
semanticscholar +2 more sources
A Structured Viroid RNA Serves as a Substrate for Dicer-Like Cleavage To Produce Biologically Active Small RNAs but Is Resistant to RNA-Induced Silencing Complex-Mediated Degradation [PDF]
Journal of Virology, 2007 A. Itaya +9 more
semanticscholar +2 more sources
Tryptophan As a New Member of RNA‐Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis
Advanced ScienceAbstractEssential amino acids (EAA) and microRNAs (miRs) control biological activity of a cell. Whether EAA regulates the activity of miR has never been demonstrated. Here, as proof‐of‐concept, a tryptophan (Trp, an EAA) complex containing Argonaute 2 (Ago2) and miRs including miR‐193a (Trp/Ago2/miR‐193a) is identified.
Fangyi Xu +11 more
openaire +4 more sources