Increased RNA-induced silencing complex (RISC) activity contributes to hepatocellular carcinoma [PDF]
Hepatology, 2011 Abstract There is virtually no effective treatment for advanced hepatocellular carcinoma (HCC) and novel targets need to be identified to develop effective treatment. We recently documented that the oncogene Astrocyte elevated gene-1 (AEG-1) plays a seminal role in hepatocarcinogenesis ...
Byoung Kwon Yoo +8 more
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Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody [PDF]
Journal of Immunological Methods, 2006 MicroRNAs (miRNAs) are short RNA molecules responsible for post-transcriptional gene silencing by the degradation or translational inhibition of their target messenger RNAs (mRNAs). This process of gene silencing, known as RNA interference (RNAi), is mediated by highly conserved Argonaute (Ago) proteins which are the key components of the RNA induced ...
Keigo Ikeda +5 more
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A 5′-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation [PDF]
Silence, 2011 MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U).Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5'-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo ...
Hervé Seitz +2 more
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Life of RISC: Formation, action, and degradation of RNA-induced silencing complex
Molecular Cell, 2021 Small RNAs regulate a wide variety of biological processes by repressing the expression of target genes at the transcriptional and post-transcriptional levels. To achieve these functions, small RNAs form RNA-induced silencing complex (RISC) together with a member of the Argonaute (AGO) protein family.
Hiro‐oki Iwakawa, Yukihide Tomari
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MicroRNAs are tightly associated with RNA-induced gene silencing complexes in vivo
Biochemical and Biophysical Research Communications, 2008 Previous work has shown that synthesized siRNA/miRNA is tightly associated with RNA-induced Gene Silencing Complexes (RISCs) in vitro. However, it is unknown if the endogenous miRNAs are also stably bound to RISC complexes in vivo in cells under physiological conditions.
Fuchou Tang +6 more
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The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts [PDF]
Genes & Development, 2016 Small RNAs regulate chromatin modification and transcriptional gene silencing across the eukaryotic kingdom. Although these processes have been well studied, fundamental mechanistic aspects remain obscure. Specifically, it is unclear exactly how small RNA-loaded Argonaute protein complexes target chromatin to mediate silencing.
Yukiko Shimada, Fabio Mohn, Marc Bühler
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PLoS ONE, 2010 GW/P bodies are cytoplasmic ribonucleoprotein-rich foci involved in microRNA (miRNA)-mediated messenger RNA (mRNA) silencing and degradation. The mRNA regulatory functions within GW/P bodies are mediated by GW182 and its binding partner hAgo2 that bind miRNA in the RNA-induced silencing complex (RISC).
Joanna J. Moser, Marvin J. Fritzler
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High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2. [PDF]
Cell Death Dis, 2018 Cappella M +11 more
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RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators [PDF]
Proceedings of the National Academy of Sciences, 2013 The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including protein kinase RNA activator (PACT), transactivation response RNA binding protein (TRBP), and Dicer, that process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation.
Andrew Redfern +20 more
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Oomycete small RNAs invade the plant RNA-induced silencing complex for virulence [PDF]
, 2019 AbstractFungal small RNAs (sRNAs) hijack the plant RNA silencing pathway to manipulate host gene expression, named cross-kingdom RNA interference (ckRNAi). It is currently unknown how conserved and significant ckRNAi is for microbial virulence. Here, we found for the first time that sRNAs of a pathogen representing the oomycete kingdom invade the host ...
Florian Dunker +8 more
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