Frontiers in Plant Science, 2022 RNA-seq has become a state-of-the-art technique for transcriptomic studies. Advances in both RNA-seq techniques and the corresponding analysis tools and pipelines have unprecedently shaped our understanding in almost every aspects of plant sciences ...
Min Tu +4 more
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Journal of Lipid Research, 2023 Small noncoding RNAs (sncRNAs) play diverse roles in numerous biological processes. While the widely used RNA sequencing (RNA-Seq) method has advanced sncRNA discovery, RNA modifications can interfere with the complementary DNA library construction ...
Rebecca Hernandez +8 more
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Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens. [PDF]
, 2015 BackgroundTo determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.MethodsTen CLL specimens and five normal peripheral blood CD19+ B ...
A Ameur +72 more
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RNA-Seq: revelation of the messengers [PDF]
Trends in Plant Science, 2013 Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists.
Van Verk +6 more
openaire +4 more sources
PLIT: An alignment-free computational tool for identification of long non-coding RNAs in plant transcriptomic datasets [PDF]
, 2019 Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs which play a significant role in several biological processes. RNA-seq based transcriptome sequencing has been extensively used for identification of lncRNAs.
Deshpande, Sumukh +4 more
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RNA-SEQ: A GLANCE AT TECHNOLOGIES AND METHODOLOGIES
Acta Biológica Colombiana, 2015 RNA Sequencing (RNA-Seq) is a newly born tool that has revolutionized the post-genomic era. The data produced by RNA-Seq, sequencing technologies and use of bioinformatics are exploding rapidly.
Seyed Mehdi Jazayeri +2 more
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RNA-seq transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis [PDF]
, 2014 Bovine tuberculosis, caused by infection with Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries.
Browne, JA +12 more
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Long read isoform sequencing reveals hidden transcriptional complexity between cattle subspecies
BMC Genomics, 2023 The Iso-Seq method of full-length cDNA sequencing is suitable to quantify differentially expressed genes (DEGs), transcripts (DETs) and transcript usage (DTU).
Yan Ren +5 more
doaj +1 more source
Deep RNA-Seq profile reveals biodiversity, plant-microbe interactions and a large family of NBS-LRR resistance genes in walnut (Juglans regia) tissues. [PDF]
, 2016 Deep RNA-Seq profiling, a revolutionary method used for quantifying transcriptional levels, often includes non-specific transcripts from other co-existing organisms in spite of stringent protocols. Using the recently published walnut genome sequence as a
Britton, Monica +3 more
core +2 more sources
Genetic circuit characterization and debugging using RNA‐seq
Molecular Systems Biology, 2017 Genetic circuits implement computational operations within a cell. Debugging them is difficult because their function is defined by multiple states (e.g., combinations of inputs) that vary in time.
Thomas E Gorochowski +7 more
doaj +1 more source