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RNA-seq

2023
We try to establish a standardized practices for RNA diagnostics using clinically accessible whole blood, we evaluated the two protocols (ribo-minus and the modified polyA+) by comparing the expression profiles and alternative splicing events of OMIM genes in clinical settings where RNA samples were collected primarily from patients whose whole genome ...
openaire   +1 more source

RNA-Seq unleashed

Nature Biotechnology, 2011
A transcriptome can be readily assembled from RNA-Seq data in the absence of a reference genome.
Matthew K Iyer, Arul M Chinnaiyan
openaire   +1 more source

Transcriptome Sequencing: RNA-Seq

2018
RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of ...
Hong, Zhang, Lin, He, Lei, Cai
openaire   +2 more sources

Complete Transcriptome RNA-Seq

2016
RNA-Seq is the leading technology for analyzing gene expression on a global scale across a broad spectrum of sample types. However, due to chemical modifications by fixation or degradation due to collection methods, samples often contain an abundance of RNA that is no longer intact, and the capability of current RNA-Seq protocols to accurately quantify
David F B, Miller   +16 more
openaire   +2 more sources

Analysis RNA-seq and Noncoding RNA

2016
RNA-Seq is an approach to transcriptome profiling that uses deep-sequencing technologies to detect and accurately quantify RNA molecules originating from a genome at a given moment in time. In recent years, the advent of RNA-Seq has facilitated genome-wide expression profiling, including the identification of novel and rare transcripts like noncoding ...
A. Arrigoni   +6 more
openaire   +3 more sources

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