Results 31 to 40 of about 345,547 (215)
Prime-seq, efficient and powerful bulk RNA sequencing
Genome Biology, 2022 Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method.
Aleksandar Janjic +11 more
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A benchmark for RNA-seq quantification pipelines [PDF]
Genome Biology, 2016 Obtaining RNA-seq measurements involves a complex data analytical process with a large number of competing algorithms as options. There is much debate about which of these methods provides the best approach. Unfortunately, it is currently difficult to evaluate their performance due in part to a lack of sensitive assessment metrics.
Michael I. Love +14 more
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Diabetes, Metabolic Syndrome and Obesity, 2023 Yue-Ling Peng,1 Yan Zhang,1 Lin Pang,2 Ya-Fang Dong,3 Mu-Ye Li,4 Hui Liao,5 Rong-Shan Li1 1Department of Nephrology, Shanxi Provincial People’s Hospital (Fifth Hospital of Shanxi Medical University), Taiyuan, People’s Republic of China; 2Department of ...
Peng YL +6 more
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RNA-seq: from technology to biology [PDF]
Cellular and Molecular Life Sciences, 2009 Next-generation sequencing technologies are now being exploited not only to analyse static genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these powerful and rapidly evolving technologies have only been available for a couple of years, they are already making substantial contributions to our understanding of genome ...
Marguerat, S, Bähler, J
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Pharmaceutics, 2016 Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq) is an emerging tool for profiling gene expression and has received ...
Joshua Xu +5 more
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Traditional versus 3′ RNA-seq in a non-model species
Genomics Data, 2017 One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript,
Sophie Tandonnet +1 more
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Probe Region Expression Estimation for RNA-Seq Data for Improved Microarray Comparability. [PDF]
PLoS ONE, 2015 Rapidly growing public gene expression databases contain a wealth of data for building an unprecedentedly detailed picture of human biology and disease.
Karolis Uziela, Antti Honkela
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PLoS ONE, 2019 The wealth of information deliverable from transcriptome sequencing (RNA-seq) is significant, however current applications for variant detection still remain a challenge due to the complexity of the transcriptome.
Modupeore O Adetunji +3 more
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COEX-Seq: Convert a Variety of Measurements of Gene Expression in RNA-Seq [PDF]
Genomics & Informatics, 2018 Next generation sequencing (NGS), a high-throughput DNA sequencing technology, is widely used for molecular biological studies. In NGS, RNA-sequencing (RNA-Seq), which is a short-read massively parallel sequencing, is a major quantitative transcriptome ...
Sang Cheol Kim +2 more
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