Results 31 to 40 of about 567,059 (348)

On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments [PDF]

, 2020
Background: In gene expression studies, RNA sample pooling is sometimes considered because of budget constraints or lack of sufficient input material. Using microarray technology, RNA sample pooling strategies have been reported to optimize both the cost
Assefa, Alemu Takele, Thas, Olivier, Vandesompele, Jo   +2 more
core   +2 more sources

Uncovering the Complexity of Transcriptomes with RNA-Seq [PDF]

Journal of Biomedicine and Biotechnology, 2010
In recent years, the introduction of massively parallel sequencing platforms for Next Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred thousand DNA fragments, dramatically changed the landscape of the genetics studies.
Costa V, Angelini C, De Feis I, Ciccodicola A   +3 more
openaire   +5 more sources

Improving RNA-Seq Precision with MapAl [PDF]

Frontiers in Genetics, 2012
With currently available RNA-Seq pipelines, expression estimates for most genes are very noisy. We here introduce MapAl, a tool for RNA-Seq expression profiling that builds on the established programs Bowtie and Cufflinks. In the post-processing of RNA-Seq reads, it incorporates gene models already at the stage of read alignment, increasing the number ...
Paweł P Łabaj, Bryan E Linggi, H Steven eWiley, David P Kreil, David P Kreil   +4 more
openaire   +4 more sources

intePareto: an R package for integrative analyses of RNA-Seq and ChIP-Seq data

BMC Genomics, 2020
Background RNA-Seq, the high-throughput sequencing (HT-Seq) of mRNAs, has become an essential tool for characterizing gene expression differences between different cell types and conditions.
Yingying Cao, Simo Kitanovski, Daniel Hoffmann   +2 more
doaj   +1 more source

Whole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis infection in vitro [PDF]

, 2013
BACKGROUND: Mycobacterium bovis, the causative agent of bovine tuberculosis, is an intracellular pathogen that can persist inside host macrophages during infection via a diverse range of mechanisms that subvert the host immune response.
Browne, JA, Conlon, KM, Gordon, SV, Gormley, E, Hokamp, K, Killick, KE, Loftus, BJ, Lohan, AJ, MacHugh, DE, Magee, DA, Nalpas, NC, Park, SD, Rue-Albrecht, K, Taraktsoglou, M   +13 more
core   +2 more sources

RNA-seq and analysis

, 2022
RNA-seq data of T24 cells in the NC and si-Livin ...
openaire   +1 more source

Advancing transcriptome platforms [PDF]

, 2011
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
core   +2 more sources

RNA-Seq Transcriptomic Profiling

, 2022
This protocol describes the preparation of RNA samples from cell lines generated at CeMM for Illumina based high-throughput sequencing using polyA-enriched library preparation.
Viollet, Coralie, Santacruz, Diana, Knebel, Dagmar, Rust, Werner, Acker, Susanne, Dick, Alec   +5 more
openaire   +2 more sources

Integrated Analysis of Single-Cell RNA-Seq and Bulk RNA-Seq Combined with Multiple Machine Learning Identified a Novel Immune Signature in Diabetic Nephropathy

Diabetes, Metabolic Syndrome and Obesity, 2023
Yue-Ling Peng,1 Yan Zhang,1 Lin Pang,2 Ya-Fang Dong,3 Mu-Ye Li,4 Hui Liao,5 Rong-Shan Li1 1Department of Nephrology, Shanxi Provincial People’s Hospital (Fifth Hospital of Shanxi Medical University), Taiyuan, People’s Republic of China; 2Department of ...
Peng YL, Zhang Y, Pang L, Dong YF, Li MY, Liao H, Li RS   +6 more
doaj  

3′ RNA-seq is superior to standard RNA-seq in cases of sparse data but inferior at identifying toxicity pathways in a model organism

Frontiers in Bioinformatics, 2023
Introduction: The application of RNA-sequencing has led to numerous breakthroughs related to investigating gene expression levels in complex biological systems.
Ryan S. McClure, Yvonne Rericha, Katrina M. Waters, Katrina M. Waters, Robyn L. Tanguay   +4 more
doaj   +1 more source