Results 31 to 40 of about 582,722 (350)

Whole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis infection in vitro [PDF]

, 2013
BACKGROUND: Mycobacterium bovis, the causative agent of bovine tuberculosis, is an intracellular pathogen that can persist inside host macrophages during infection via a diverse range of mechanisms that subvert the host immune response.
Browne, JA, Conlon, KM, Gordon, SV, Gormley, E, Hokamp, K, Killick, KE, Loftus, BJ, Lohan, AJ, MacHugh, DE, Magee, DA, Nalpas, NC, Park, SD, Rue-Albrecht, K, Taraktsoglou, M   +13 more
core   +2 more sources

Uncovering the Complexity of Transcriptomes with RNA-Seq [PDF]

Journal of Biomedicine and Biotechnology, 2010
In recent years, the introduction of massively parallel sequencing platforms for Next Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred thousand DNA fragments, dramatically changed the landscape of the genetics studies.
Costa V, Angelini C, De Feis I, Ciccodicola A   +3 more
openaire   +4 more sources

Integrated Analysis of Single-Cell RNA-Seq and Bulk RNA-Seq Combined with Multiple Machine Learning Identified a Novel Immune Signature in Diabetic Nephropathy

Diabetes, Metabolic Syndrome and Obesity, 2023
Yue-Ling Peng,1 Yan Zhang,1 Lin Pang,2 Ya-Fang Dong,3 Mu-Ye Li,4 Hui Liao,5 Rong-Shan Li1 1Department of Nephrology, Shanxi Provincial People’s Hospital (Fifth Hospital of Shanxi Medical University), Taiyuan, People’s Republic of China; 2Department of ...
Peng YL, Zhang Y, Pang L, Dong YF, Li MY, Liao H, Li RS   +6 more
doaj  

Case study: Targeted RNA-sequencing of aged formalin-fixed paraffin-embedded samples for understanding chemical mode of action

Toxicology Reports, 2022
Formalin-fixed paraffin-embedded (FFPE) samples are the only remaining biological archive for many toxicological and clinical studies, yet their use in genomics has been limited due to nucleic acid damage from formalin fixation.
Michael D. Cannizzo, Charles E. Wood, Susan D. Hester, Leah C. Wehmas   +3 more
doaj   +1 more source

Comprehensive Assessments of RNA-seq by the SEQC Consortium: FDA-Led Efforts Advance Precision Medicine

Pharmaceutics, 2016
Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq) is an emerging tool for profiling gene expression and has received ...
Joshua Xu, Binsheng Gong, Leihong Wu, Shraddha Thakkar, Huixiao Hong, Weida Tong   +5 more
doaj   +1 more source

MINTmap: fast and exhaustive profiling of nuclear and mitochondrial tRNA fragments from short RNA-seq data. [PDF]

, 2017
Transfer RNA fragments (tRFs) are an established class of constitutive regulatory molecules that arise from precursor and mature tRNAs. RNA deep sequencing (RNA-seq) has greatly facilitated the study of tRFs.
Loher, Phillipe, Rigoutsos, Isidore, Telonis, Aristeidis G.   +2 more
core   +2 more sources

Traditional versus 3′ RNA-seq in a non-model species

Genomics Data, 2017
One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript,
Sophie Tandonnet, Tatiana Teixeira Torres   +1 more
doaj   +1 more source

MSIQ: Joint Modeling of Multiple RNA-seq Samples for Accurate Isoform Quantification

, 2017
Next-generation RNA sequencing (RNA-seq) technology has been widely used to assess full-length RNA isoform abundance in a high-throughput manner. RNA-seq data offer insight into gene expression levels and transcriptome structures, enabling us to better ...
Li, Jingyi Jessica, Li, Wei Vivian, Zhang, Shihua, Zhao, Anqi   +3 more
core   +2 more sources

Advancing transcriptome platforms [PDF]

, 2011
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
core   +2 more sources

Improving RNA-Seq Precision with MapAl [PDF]

Frontiers in Genetics, 2012
With currently available RNA-Seq pipelines, expression estimates for most genes are very noisy. We here introduce MapAl, a tool for RNA-Seq expression profiling that builds on the established programs Bowtie and Cufflinks. In the post-processing of RNA-Seq reads, it incorporates gene models already at the stage of read alignment, increasing the number ...
Paweł P Łabaj, Bryan E Linggi, H Steven eWiley, David P Kreil, David P Kreil   +4 more
openaire   +3 more sources