Results 41 to 50 of about 345,547 (215)
Design, execution, and interpretation of plant RNA-seq analyses
Frontiers in Plant Science, 2023 Genomics has transformed our understanding of the genetic architecture of traits and the genetic variation present in plants. Here, we present a review of how RNA-seq can be performed to tackle research challenges addressed by plant sciences.
Racheal N. Upton +6 more
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trumpet: transcriptome-guided quality assessment of m6A-seq data
BMC Bioinformatics, 2018 Background Methylated RNA immunoprecipitation sequencing (MeRIP-seq or m6A-seq) has been extensively used for profiling transcriptome-wide distribution of RNA N6-Methyl-Adnosine methylation.
Teng Zhang +3 more
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Polyribosomal RNA-Seq reveals the decreased complexity and diversity of the Arabidopsis translatome.
PLoS ONE, 2015 Recent RNA-seq studies reveal that the transcriptomes in animals and plants are more complex than previously thought, leading to the inclusion of many more splice isoforms in annotated genomes. However, it is possible that a significant proportion of the
Xingtan Zhang +4 more
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Journal of Lipid Research, 2023 Small noncoding RNAs (sncRNAs) play diverse roles in numerous biological processes. While the widely used RNA sequencing (RNA-Seq) method has advanced sncRNA discovery, RNA modifications can interfere with the complementary DNA library construction ...
Rebecca Hernandez +8 more
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RNA‐Seq for Bacterial Gene Expression [PDF]
Current Protocols in Nucleic Acid Chemistry, 2018 AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
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L-GIREMI uncovers RNA editing sites in long-read RNA-seq
Genome Biology, 2023 Although long-read RNA-seq is increasingly applied to characterize full-length transcripts it can also enable detection of nucleotide variants, such as genetic mutations or RNA editing sites, which is significantly under-explored.
Zhiheng Liu +7 more
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BioengineeringRNA sequencing (RNA-seq) has emerged as a prominent resource for transcriptomic analysis due to its ability to measure gene expression in a highly sensitive and accurate manner.
Farhana Manzoor +2 more
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VIPER: Visualization Pipeline for RNA-seq, a Snakemake workflow for efficient and complete RNA-seq analysis [PDF]
BMC Bioinformatics, 2018 RNA sequencing has become a ubiquitous technology used throughout life sciences as an effective method of measuring RNA abundance quantitatively in tissues and cells. The increase in use of RNA-seq technology has led to the continuous development of new tools for every step of analysis from alignment to downstream pathway analysis. However, effectively
Henry W. Long +15 more
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PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data
Genomics, Proteomics & Bioinformatics, 2014 The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and changes in alternative splicing (AS) can impact gene function or cause disease.
Collin Tokheim, Juw Won Park, Yi Xing
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