Results 51 to 60 of about 345,547 (215)

Development and evaluation of RNA-seq methods [PDF]

Genome Biology, 2010
This article is part of the supplement: Beyond the Genome: The true gene count, human evolution and disease ...
Nir Friedman, Lin Fan, Xian Adiconis, Andreas Gnirke, Dawn Thompson, Moran Yassour, Moran Yassour, Mitchell Guttman, Michael F. Berger, Aviv Regev, Chad Nusbaum, Joshua Z. Levin   +11 more
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Dissecting intratumoral myeloid cell plasticity by single cell RNA‐seq

Cancer Medicine, 2019
Tumor‐infiltrating myeloid cells are the most abundant leukocyte population within tumors. Molecular cues from the tumor microenvironment promote the differentiation of immature myeloid cells toward an immunosuppressive phenotype.
Qianqian Song, Gregory A. Hawkins, Leonard Wudel, Ping‐Chieh Chou, Elizabeth Forbes, Ashok K. Pullikuth, Liang Liu, Guangxu Jin, Lou Craddock, Umit Topaloglu, Gregory Kucera, Stacey O’Neill, Edward A. Levine, Peiqing Sun, Kounosuke Watabe, Yong Lu, Martha A. Alexander‐Miller, Boris Pasche, Lance D. Miller, Wei Zhang   +19 more
doaj   +1 more source

Comparison of RNA-Seq and microarray in transcriptome profiling of activated T cells.

PLoS ONE, 2014
To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, both RNA-Seq and microarray analyses were performed on RNA samples from a human T cell activation experiment.
Shanrong Zhao, Wai-Ping Fung-Leung, Anton Bittner, Karen Ngo, Xuejun Liu   +4 more
doaj   +1 more source

DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate

Plant Methods, 2022
Background Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the last decade, RNA extraction remains ...
Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata, Atsushi J. Nagano   +6 more
doaj   +1 more source

Evaluation and application of RNA-Seq by MinION [PDF]

DNA Research, 2018
The current RNA-Seq method analyses fragments of mRNAs, from which it is occasionally difficult to reconstruct the entire transcript structure. Here, we performed and evaluated the recent procedure for full-length cDNA sequencing using the Nanopore sequencer MinION.
Martin C. Frith, Martin C. Frith, Eri Katsumata, Masahide Seki, Yutaka Suzuki, Yoshitaka Sakamoto, Sarun Sereewattanawoot, Sumio Sugano, Sumio Sugano, Katsuya Tsuchihara, Takashi Kohno, Ayako Suzuki, Junko Mizushima-Sugano, Junko Mizushima-Sugano   +13 more
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A Novel Combinatorial Method for Estimating Transcript Expression with RNA-Seq: Bounding the Number of Paths [PDF]

arXiv, 2013
RNA-Seq technology offers new high-throughput ways for transcript identification and quantification based on short reads, and has recently attracted great interest. The problem is usually modeled by a weighted splicing graph whose nodes stand for exons and whose edges stand for split alignments to the exons.
arxiv  

Comparison of RNA-Seq and Microarray Gene Expression Platforms for the Toxicogenomic Evaluation of Liver From Short-Term Rat Toxicity Studies

Frontiers in Genetics, 2019
Gene expression profiling is a useful tool to predict and interrogate mechanisms of toxicity. RNA-Seq technology has emerged as an attractive alternative to traditional microarray platforms for conducting transcriptional profiling.
Mohan S. Rao, Terry R. Van Vleet, Rita Ciurlionis, Wayne R. Buck, Scott W. Mittelstadt, Eric A. G. Blomme, Michael J. Liguori   +6 more
doaj   +1 more source

LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis

Genome Biology, 2023
Existing single-cell RNA sequencing (scRNA-seq) methods rely on reverse transcription (RT) and second-strand synthesis (SSS) to convert single-stranded RNA into double-stranded DNA prior to amplification, with the limited RT/SSS efficiency compromising ...
Jun Lyu, Chongyi Chen
doaj   +1 more source

Supersplat—spliced RNA-seq alignment [PDF]

Bioinformatics, 2010
Abstract Motivation: High-throughput sequencing technologies have recently made deep interrogation of expressed transcript sequences practical, both economically and temporally. Identification of intron/exon boundaries is an essential part of genome annotation, yet remains a challenge.
Rongkun Shen, Weng-Keen Wong, Henry D. Priest, Todd C. Mockler, Douglas W. Bryant   +4 more
openaire   +3 more sources

KIF2C affects sperm cell differentiation in patients with Klinefelter syndrome, as revealed by RNA‐Seq and scRNA‐Seq data

FEBS Open Bio, 2022
Klinefelter syndrome (KS) is a leading contributor to male infertility and is characterised by complex and diverse clinical features; however, genetic changes in the KS transcriptome remain largely unknown.
Haihong He, Tingting Huang, Fan Yu, Keyan Chen, Shixing Guo, Lijun Zhang, Xi Tang, Xinhua Yuan, Jiao Liu, Yiwen Zhou   +9 more
doaj   +1 more source