Results 51 to 60 of about 536,139 (245)
Design, execution, and interpretation of plant RNA-seq analyses
Frontiers in Plant Science, 2023 Genomics has transformed our understanding of the genetic architecture of traits and the genetic variation present in plants. Here, we present a review of how RNA-seq can be performed to tackle research challenges addressed by plant sciences.
Racheal N. Upton +6 more
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Traditional versus 3′ RNA-seq in a non-model species
Genomics Data, 2017 One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript,
Sophie Tandonnet +1 more
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Journal of Lipid Research, 2023 Small noncoding RNAs (sncRNAs) play diverse roles in numerous biological processes. While the widely used RNA sequencing (RNA-Seq) method has advanced sncRNA discovery, RNA modifications can interfere with the complementary DNA library construction ...
Rebecca Hernandez +8 more
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trumpet: transcriptome-guided quality assessment of m6A-seq data
BMC Bioinformatics, 2018 Background Methylated RNA immunoprecipitation sequencing (MeRIP-seq or m6A-seq) has been extensively used for profiling transcriptome-wide distribution of RNA N6-Methyl-Adnosine methylation.
Teng Zhang +3 more
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L-GIREMI uncovers RNA editing sites in long-read RNA-seq
Genome Biology, 2023 Although long-read RNA-seq is increasingly applied to characterize full-length transcripts it can also enable detection of nucleotide variants, such as genetic mutations or RNA editing sites, which is significantly under-explored.
Zhiheng Liu +7 more
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PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data
Genomics, Proteomics & Bioinformatics, 2014 The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and changes in alternative splicing (AS) can impact gene function or cause disease.
Collin Tokheim, Juw Won Park, Yi Xing
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Simultaneous generation of many RNA-seq libraries in a single reaction [PDF]
, 2015 Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications.
Bhattacharyya, Roby P. +15 more
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Comparison of RNA-Seq and microarray in transcriptome profiling of activated T cells.
PLoS ONE, 2014 To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, both RNA-Seq and microarray analyses were performed on RNA samples from a human T cell activation experiment.
Shanrong Zhao +4 more
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Identification of rare-disease genes using blood transcriptome sequencing and large control cohorts. [PDF]
, 2019 It is estimated that 350 million individuals worldwide suffer from rare diseases, which are predominantly caused by mutation in a single gene1. The current molecular diagnostic rate is estimated at 50%, with whole-exome sequencing (WES) among the most ...
Ashley, Euan A +35 more
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