Results 71 to 80 of about 5,194,805 (386)
Purification tags markedly affect self‐aggregation of CPEB3
FEBS Letters, EarlyView.Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito +6 more
wiley +1 more source
Transfer RNA and Origins of RNA Interference [PDF]
Frontiers in Molecular Biosciences, 2021 Almost 30 years ago the first microRNA (miRNA) was detected (Lee et al., 1993), later put in mechanistical context by the discovery of the RNA interference (RNAi) and RNA-induced silencing complex (RISC). miRNA is the variable RISC element (guide) that recognizes messenger RNA (mRNA) targets in the RNAi process facilitated by Argonaute (Ago) proteins ...
openaire +5 more sources
Nucleic Acids Res., 2013 Although microRNAs (miRNAs), other non-coding RNAs (ncRNAs) (e.g. lncRNAs, pseudogenes and circRNAs) and competing endogenous RNAs (ceRNAs) have been implicated in cell-fate determination and in various human diseases, surprisingly little is known about ...
Junhao Li +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source
A scaling normalization method for differential expression analysis of RNA-seq data
Genome Biology, 2010 The fine detail provided by sequencing-based transcriptome surveys suggests that RNA-seq is likely to become the platform of choice for interrogating steady state RNA.
Mark D Robinson, Alicia Oshlack
semanticscholar +1 more source
Current Opinion in Structural Biology, 1992 RNA pseudoknots result from Watson-Crick base pairing involving a stretch of bases located between paired strands and a distal single-stranded region. Recently, significant advances in our understanding of their structural and functional aspects have been accomplished.
Westhof, Eric, Jaeger, Luc
openaire +1 more source
RILogo: visualizing RNA–RNA interactions [PDF]
Bioinformatics, 2012 Abstract Summary: With the increasing amount of newly discovered non-coding RNAs, the interactions between RNA molecules become an increasingly important aspect for characterizing their functionality. Many computational tools have been developed to predict the formation of duplexes between two RNAs, either based on single sequences or ...
Menzel, Karl Peter +2 more
openaire +4 more sources
Infection and Drug Resistance, 2023 Jinjuan Zhang,1 Caifang Bu,1 Liwei Yao,1,* Kan Xu2,* 1Department of Nursing, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People’s Republic of China; 2Zhejiang Tuberculosis Diagnosis and ...
Zhang J, Bu C, Yao L, Xu K
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The 5'-3' exoribonuclease Pacman (Xrn1) regulates expression of the heat shock protein Hsp67Bc and the microRNA miR-277-3p in Drosophila wing imaginal discs [PDF]
, 2013 Pacman/Xrn1 is a highly conserved exoribonuclease known to play a critical role in gene regulatory events such as control of mRNA stability, RNA interference and regulation via miRNAs.
Bate M +7 more
core +1 more source
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Nucleic Acids Research, 2007 The publication of a complete genome sequence is usually accompanied by annotations of its genes. In contrast to protein coding genes, genes for ribosomal RNA (rRNA) are often poorly or inconsistently annotated.
K. Lagesen +5 more
semanticscholar +1 more source