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Purification of RNA-Binding Proteins

2014
The majority of regulatory RNA sequences exert their function through interaction with proteins. Therefore, the identification of RNA-binding proteins is the key step in understanding the role of many RNA motifs. Here, we describe a straightforward method to identify RNA-binding proteins.
Birgit, Ritter, Marc R, Reboll
openaire   +2 more sources

RNA-binding proteins in RNA interference

Molecular Biology, 2006
Short RNAs (21–27 nt) silence genes that contain homologous nucleotide sequences; this is known as RNA silencing. This review considers the generation of short RNAs from their precursors: double-stranded RNAs, capable of inducing RNA interference, and hairpin RNAs, whose processing yields microRNAs, as well as the properties of RNA-binding domains that
R. N. Kotelnikov   +3 more
openaire   +1 more source

Polypyrimidine-tract-binding protein: a multifunctional RNA-binding protein

Biochemical Society Transactions, 2008
PTB (polypyrimidine-tract-binding protein) is a ubiquitous RNA-binding protein. It was originally identified as a protein with a role in splicing but it is now known to function in a large number of diverse cellular processes including polyadenylation, mRNA stability and translation initiation.
Kirsty, Sawicka   +3 more
openaire   +2 more sources

The RNA-binding proteins

2015
This chapter details how RNA-binding proteins package RNA, protect RNA, organize RNA, and prepare RNA for post-transcriptional processes. It describes different kinds of RNA-binding and auxiliary domains that enable RNA-binding proteins to bind RNA in a versatile way.
David Elliott, Michael Ladomery
openaire   +1 more source

RNA-binding proteins mind the GAPs

Nature Immunology, 2017
Increased expression of the ubiquitin ligase TRAF6 in hematopoietic stem cells promotes activity of the GTP-binding protein Cdc42 and consequent diminished function of hematopoietic stem cells by ubiquitination of the RNA-binding protein hnRNP-A1, which leads to an inability to properly process pre-mRNA encoding Cdc42-inhibitory GTPase-activating ...
Martin Turner, Elisa Monzón-Casanova
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Structures of RNA-binding proteins

Quarterly Reviews of Biophysics, 1997
J G, Arnez, J, Cavarelli
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RNA-Binding Proteins (RBPs) and Circular RNA Biogenesis

Many protein-coding genes in eukaryotes have the capacity to produce circular RNAs (circRNAs). CircRNAs are stable and covalently closed products generated from backsplicing. Their biogenesis is regulated by multiple factors, with RNA-binding proteins (RBPs) playing a critical role. RBPs bind to flanking introns of the circularizing exons, bringing the
Shasha, Shi, Rui, Zhao
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The kinetic landscape of an RNA-binding protein in cells

Nature, 2021
Matthew M Brister   +2 more
exaly  

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