Results 251 to 260 of about 134,303 (299)

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Precision Chemistry for Protein Lysine Modification

Chemistry – A European Journal, EarlyView.
Selective modification of lysine residues is challenging due to their similar intrinsic reactivity. Inspired by enzymatic recognition, ligand‐guided electrophiles enable site‐selective labeling and functionalization, while ligand‐guided catalyses achieve regioselective installation of bio‐relevant post‐translational modifications.
Mayu Onoda, Motomu Kanai
wiley   +1 more source

Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress. [PDF]

Sci Adv
Mazzola MC, Zhao T, Kiem A, Kristiansen TA, Gustafsson K, Wong LP, Scott-Solomon E, Fahlberg MD, Mayerhofer C, Mayerhofer E, Forward S, Assita ER, Schiroli G, Handley M, Kfoury Y, Fukushima T, Li D, Keyes S, Sharda A, Milosevic J, Kato H, Ivanov P, Sykes DB, Kwok SJJ, Sadreyev RI, Sankaran VG, Hsu YC, Scadden DT.   +27 more
europepmc   +1 more source

An Electrophilic Uridine Building Block for Post‐Synthetic RNA Modification as Exemplified for Spin Labeling

Chemistry – A European Journal, EarlyView.
An electrophilic 5‐isothiocyanatomethyl uridine building block enables modular post‐synthetic RNA labeling with primary amine‐containing probes. Positioning the functionality in the major groove minimizes structural perturbation and allows facile conjugation of sensitive tags such as TEMPO spin labels for paramagnetic relaxation enhancement studies ...
Lisa Ruetz, Sonja Leiner, Alessandro Marotto, Raphael Plangger, Christoph Kreutz   +4 more
wiley   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Click to Translate: Synthesis of Trans-Cyclooctene Modified 5' mRNA Caps for Bioorthogonal Activation. [PDF]

Chembiochem
Zips N, Kulko E, Kath-Schorr S.
europepmc   +1 more source

Engineered T7 RNA polymerase to improve mRNA capping efficiency and reduce dsRNA generation during <i>in vitro</i> transcription. [PDF]

Mol Ther Adv
Sanjeev A, Hannigan C, Garrity R, Kamalakkannan DS, Clendaniel V, Mantri M, Glass Z, Kasiewicz LN, Leipheimer J, Khan Z, Mizoguchi T, Peshdary V, Summers CR, Beuning PJ, Cheng CJ.   +14 more
europepmc   +1 more source

Decoding Self vs. Non-Self: Alphavirus Cap0 Recognition and Immune Evasion. [PDF]

Viruses
Faraj SE, Filomatori CV.
europepmc   +1 more source

Beyond the Sequence: Chemical and Topological Design and Innovations in mRNA Therapeutics. [PDF]

Chem Rev
Liu D, Chen H, Pan A, Wang X.
europepmc   +1 more source

A self-amplifying RNA vector based on rubella virus for mRNA therapeutics and vaccine applications. [PDF]

Mol Ther Nucleic Acids
Jing M, Law MCY, Phung CD, He Y, Tan YB, Le MTN, Luo D.   +6 more
europepmc   +1 more source