Results 271 to 280 of about 134,303 (299)

Synthesis of 5′-NAD-Capped RNA

Bioconjugate Chemistry, 2016
In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5'-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5'
Höfer, K., Abele, F., Schlotthauer, J., Jäschke, A.   +3 more
openaire   +3 more sources

Transcription Activation at Class II CAP-Dependent Promoters: Two Interactions between CAP and RNA Polymerase

Cell, 1996
At Class II catabolite activator protein (CAP)-dependent promoters, CAP activates transcription from a DNA site overlapping the DNA site for RNA polymerase.
Younggyu Kim, Tomasz Heyduk, Richard H Ebright   +2 more
exaly   +2 more sources

‘Cap’ structures in maize poly(A)-containing RNA

Nucleic Acids and Protein Synthesis, 1979
Poly(A)-containing RNA was isolated from maize embryos by chromatography on columns of oligo(dT)-cellulose and exhaustively digested with ribonucleases T2, T1, and A. Fractionation of the digests by two-dimensional electrophoresis revealed the presence of three 7-methylguanosine-terminated 'cap structures' of the type m7GpppNp.
J L Nichols
exaly   +3 more sources

Good cap/bad cap: how the cap-binding complex determines RNA fate

Nature Structural & Molecular Biology, 2014
Every RNA polymerase II transcript receives a 5′-end 7-methylguanosine (m7G) cap, which is rapidly bound by the nuclear cap–binding complex (CBC). Two recent studies now reveal that the CBC associates with a variety of effector proteins that enable it to interrogate nascent RNA, discriminating between distinct RNA subclasses and routing them either ...
Müller-McNicoll, M., Neugebauer, K.
openaire   +3 more sources

A General RNA-Capping Ribozyme Retains Stereochemistry during Cap Exchange

Journal of the American Chemical Society, 2006
Numerous natural and artificial ribozymes have been shown to facilitate reactions that invert stereochemistry. Here, we demonstrate that an RNA-capping ribozyme retains stereochemistry at a phosphorus reaction center. The ribozyme synthesizes a broad range of 5'-5' RNA caps by exchanging phosphate groups around the alpha-phosphate found at the 5 ...
Hani S, Zaher, Peter J, Unrau
openaire   +2 more sources

Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein

2023
Abstract mRNA vaccines and therapeutics are highly effective and can be developed and manufactured with a relatively short lead time. Here we report an all-enzyme platform to generate capped synthetic RNA in a one-step process based on an RNA capping enzyme-T7 RNA polymerase fusion protein. Under standard in vitro transcription reaction
S. Hong Chan, Louise Tirole, Daniel Kneller, Theresa M. Kelley, Nan Dai, G. Brett Robb   +5 more
openaire   +1 more source

The uncharted territory of NAD+-capped RNA

Trends in Cell Biology
The critical redox cofactor NAD+ was recently reported to serve as an RNA cap in both eukaryotes and prokaryotes. However, its reversible regulation and biological functions remain unclear. Here, we provide insights into its discovery, capping and decapping mechanisms, for further discovery of their potential functional implications.
Quan Ma, Jianzhao Liu, Hao Hu, Zisheng Luo   +3 more
openaire   +2 more sources

The RNA of unfertilized sea urchin eggs is ‘capped’

Cell Differentiation, 1977
It is shown that the RNA of unfertilized sea urchin eggs is active in stimulating protein synthesis in a wheat germ cell free system. This activity is not lowered by conditions that inhibit the methylation processes but is inhibited by a treatment that damages the "cap", A difference in activity in a wheat germ cell free system between the RNA of ...
A M, Pirrone, G, Spinelli, P, Acierno, M, Errera, G, Giudice   +4 more
openaire   +2 more sources

Potential Therapeutic Applications of Rna Cap Analogs

Future Medicinal Chemistry, 2013
Cap analogs are chemically modified derivatives of the unique cap structure present at the 5´ end of all eukaryotic mRNAs and several non-coding RNAs. Until recently, cap analogs have served primarily as tools in the study of RNA metabolism. Continuing advances in our understanding of cap biological functions (including RNA stabilization, pre-mRNA ...
Marcin, Ziemniak, Malwina, Strenkowska, Joanna, Kowalska, Jacek, Jemielity   +3 more
openaire   +2 more sources

[Capping strategies in RNA viruses].

Medecine sciences : M/S, 2012
Most viruses use the mRNA-cap dependent cellular translation machinery to translate their mRNAs into proteins. The addition of a cap structure at the 5' end of mRNA is therefore an essential step for the replication of many virus families. Additionally, the cap protects the viral RNA from degradation by cellular nucleases and prevents viral RNA ...
Mickaël, Bouvet, François, Ferron, Isabelle, Imbert, Laure, Gluais, Barbara, Selisko, Bruno, Coutard, Bruno, Canard, Etienne, Decroly   +7 more
openaire   +1 more source