Results 71 to 80 of about 134,303 (299)

Integrative exploration of the mutual gene signatures and immune microenvironment between benign prostate hyperplasia and castration-resistant prostate cancer

The Aging Male, 2023
Background Benign prostate hyperplasia (BPH) and prostate cancer (CaP) are among the most frequently occurring prostatic diseases. When CaP progressed to castration-resistant CaP (CRPC), the prognosis is poor. Although CaP/CRPC and BPH frequently coexist
Fei Wu, Hao Ning, Yang Sun, Haihu Wu, Jiaju Lyu   +4 more
doaj   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Clinical Significance of Two Real-Time PCR Assays for Chronic Hepatitis C Patients Receiving Protease Inhibitor-Based Therapy.

PLoS ONE, 2017
The aim of this study was to determine the efficacy of two hepatitis C virus (HCV) real-time PCR assays, the COBAS AmpliPrep/COBAS TaqMan HCV test (CAP/CTM) and the Abbott RealTime HCV test (ART), for predicting the clinical outcomes of patients infected
Takako Inoue, Su Su Hmwe, Noritomo Shimada, Keizo Kato, Tatsuya Ide, Takuji Torimura, Takashi Kumada, Hidenori Toyoda, Akihito Tsubota, Koichi Takaguchi, Takaji Wakita, Yasuhito Tanaka   +11 more
doaj   +1 more source

A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation

Scientific Reports, 2021
Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE ...
Brandon M. Trainor, Arnab Ghosh, Dimitri G. Pestov, Christopher U. T. Hellen, Natalia Shcherbik   +4 more
doaj   +1 more source

Developmental programmes drive cellular plasticity, disease progression and therapy resistance in lung adenocarcinoma

Molecular Oncology, EarlyView.
This study shows that lung adenocarcinomas exploit developmental branching morphogenesis to acquire a therapy resistant basal‐like tumour cell state. This process was found to be regulated by combined TP53 loss‐of‐function and type‐I interferon signalling, identifying a novel axis for biomarker and therapeutic target discovery.
Kamila J Bienkowska, Stephany Gallardo, Nur S. Zainal, Leena Arora, Matthew Ellis, Maria‐Antoinette Lopez, Judith Austine, Sai Pittla, Serena J Chee, Aiman Alzetani, Emily C Shaw, Christian H Ottensmeier, Gareth J Thomas, Christopher J Hanley   +13 more
wiley   +1 more source

Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach [PDF]

Nucleic Acids Research, 2001
An effective procedure for specific determination of the cap structure at the 5'-terminus of mRNA and for isolation of the corresponding full-length cDNA has been developed. The procedure involves covalent attachment of an oligonucleotide template extender to the 5'-cap structure of mRNA followed by RT-PCR using M-MLV SuperScript II reverse ...
V A, Efimov, O G, Chakhmakhcheva, J, Archdeacon, J M, Fernandez, O N, Fedorkin, Y L, Dorokhov, J G, Atabekov   +6 more
openaire   +2 more sources

A splicing-dependent transcriptional checkpoint associated with prespliceosome formation

, 2014
There is good evidence for functional interactions between splicing and transcription in eukaryotes, but how and why these processes are coupled remain unknown.
Chathoth, Keerthi T, Webb, Shaun, Barrass, J David, Beggs, Jean D   +3 more
core   +1 more source

Genome-scale characterization of RNA tertiary structures and their functional impact by RNA solvent accessibility prediction [PDF]

, 2016
As most RNA structures are elusive to structure determination, obtaining solvent accessible surface areas (ASAs) of nucleotides in an RNA structure is an important first step to characterize potential functional sites and core structural regions.
Wang, Jihua, Zhan, Jian, Zhou, Yaoqi, Zhang, Jian, Yaoqi Zhou, Li, Xiaomei, Yuedong Yang, Jian Zhan, Huiying Zhao, Yang, Yuedong, Zhao, Huiying, Xiaomei Li, Jihua Wang   +12 more
core   +1 more source

Structural basis of Spliced Leader RNA recognition by the Trypanosoma brucei cap-binding complex

Nature Communications
Kinetoplastids are a clade of eukaryotic protozoans that include human parasitic pathogens like trypanosomes and Leishmania species. In these organisms, protein-coding genes are transcribed as polycistronic pre-mRNAs, which need to be processed by the ...
Harald Bernhard, Hana Petržílková, Barbora Popelářová, Kamil Ziemkiewicz, Karolina Bartosik, Marcin Warmiński, Laura Tengo, Henri Gröger, Luciano G. Dolce, Cameron D. Mackereth, Ronald Micura, Jacek Jemielity, Eva Kowalinski   +12 more
doaj   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source