Results 91 to 100 of about 134,303 (299)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RNA-Seq strand specific reads alignments on Rep/Cap cassette, 48 and 94 hours post transfection.
, 2018 In dark red the reads correspond to rep sequence alignments on the negative strand and in black, alignments of the cap sequence 48 hours post transfection.
Minna U. Kaikkonen (193048) +5 more
core +1 more source
PLoS ONEAll eukaryotic mRNAs bear a 7-methylguanosine cap on their 5' end. The 5' cap enables mRNA translation by binding directly to eIF4E; which further recruits other factors and the 40S ribosome.
Zachary F Mandell, Jeff Coller
doaj +1 more source
Frontiers in Plant Science, 2017 Most of the positive-strand RNA plant viruses lack the 5′-cap and/or the poly(A)-tail that act synergistically to stimulate canonical translation of cellular mRNAs.
Verónica Truniger +2 more
doaj +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
FEBS Open Bio, EarlyView.Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
The splicing factor proline-glutamine rich (SFPQ/PSF) is involved in influenza virus transcription.
, 2011 The influenza A virus RNA polymerase is a heterotrimeric complex responsible for viral genome transcription and replication in the nucleus of infected cells. We recently carried out a proteomic analysis of purified polymerase expressed in human cells and
Juan Ortín +15 more
core +1 more source
Translation control of Enterovirus A71 gene expression
Journal of Biomedical Science, 2020 Upon EV-A71 infection of a host cell, EV-A71 RNA is translated into a viral polyprotein. Although EV-A71 can use the cellular translation machinery to produce viral proteins, unlike cellular translation, which is cap-dependent, the viral RNA genome of EV-
Ming-Chih Lai +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source
Genome transcription/translation of segmented, negative-strand RNA viruses
, 2011 The requirements for alignment of capped RNA leader sequences along the viral genome during influenza transcription initiation (“cap-snatching”) have long been an enigma.
Geerts-Dimitriadou, C.
core
Cap-dependent translation is mediated by 'RNA looping' rather than 'ribosome scanning'
, 2018 The 40S ribosomal subunit cannot directly recognize the start codon of eukaryotic mRNAs. Instead, it recognizes the start codon after its association with the 5 '-cap structure via translation initiation factors.
Jang, SK, Paek, KY
core +1 more source