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NAD-capped RNAs – a redox cofactor meets RNA
Trends in Biochemical Sciences, 2023RNA modifications immensely expand the diversity of the transcriptome, thereby influencing the function, localization, and stability of RNA. One prominent example of an RNA modification is the eukaryotic cap located at the 5' terminus of mRNAs. Interestingly, the redox cofactor NAD can be incorporated into RNA by RNA polymerase in vitro.
Maik Wolfram-Schauerte, Katharina Höfer
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Synthesis of 5′-NAD-Capped RNA
Bioconjugate Chemistry, 2016In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5'-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5'
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