Results 181 to 190 of about 28,766 (215)
Some of the next articles are maybe not open access.
Good cap/bad cap: how the cap-binding complex determines RNA fate
Nature Structural & Molecular Biology, 2014Every RNA polymerase II transcript receives a 5′-end 7-methylguanosine (m7G) cap, which is rapidly bound by the nuclear cap–binding complex (CBC). Two recent studies now reveal that the CBC associates with a variety of effector proteins that enable it to interrogate nascent RNA, discriminating between distinct RNA subclasses and routing them either ...
Müller-McNicoll, M., Neugebauer, K.
openaire +3 more sources
Chemical Route to the Capped RNAs
Nucleosides, Nucleotides & Nucleic Acids, 2004Eukaryotic and viral messenger RNAs contain a CAP structure that plays an important role in the initiation of translation and several other cellular processes that involve mRNAs. In this paper, we report a convenient chemical approach to the preparation of milligram quantities of short, capped RNA oligonucleotides, which overcomes some of the ...
I I, Koukhareva, A V, Lebedev
openaire +2 more sources
A General RNA-Capping Ribozyme Retains Stereochemistry during Cap Exchange
Journal of the American Chemical Society, 2006Numerous natural and artificial ribozymes have been shown to facilitate reactions that invert stereochemistry. Here, we demonstrate that an RNA-capping ribozyme retains stereochemistry at a phosphorus reaction center. The ribozyme synthesizes a broad range of 5'-5' RNA caps by exchanging phosphate groups around the alpha-phosphate found at the 5 ...
Hani S, Zaher, Peter J, Unrau
openaire +2 more sources
Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein
2023AbstractmRNA vaccines and therapeutics are highly effective and can be developed and manufactured with a relatively short lead time. Here we report an all-enzyme platform to generate capped synthetic RNA in a one-step process based on an RNA capping enzyme-T7 RNA polymerase fusion protein.
S. Hong Chan +5 more
openaire +1 more source
Potential Therapeutic Applications of Rna Cap Analogs
Future Medicinal Chemistry, 2013Cap analogs are chemically modified derivatives of the unique cap structure present at the 5´ end of all eukaryotic mRNAs and several non-coding RNAs. Until recently, cap analogs have served primarily as tools in the study of RNA metabolism. Continuing advances in our understanding of cap biological functions (including RNA stabilization, pre-mRNA ...
Marcin, Ziemniak +3 more
openaire +2 more sources
5‘-RNA Self-Capping from Guanosine Diphosphate
Biochemistry, 1997A selected RNA (isolate 6) efficiently catalyzes a self-capping reaction with free GDP, yielding the same 5'-capped structure as is formed by protein GTP:RNA guanylyltransferase. This unexplored RNA-catalyzed reaction type involving nucleophilic attack on phosphate by phosphate adds to the variety of possible postsynthetic RNA-catalyzed RNA ...
F, Huang, M, Yarus
openaire +2 more sources
Messenger RNA Capping Enzymes from Eukaryotic Cells
1987Publisher Summary This chapter discusses the messenger molecular mechanism of mRNA capping in eukaryotic cells. To understand the molecular mechanism, it is necessary to purify and characterize the enzymes involved. Most of the cellular as well as viral mRNAs in eukaryotes contain a 5´-terminal “cap” structure—m 7 GpppN—in which the terminal 7 ...
K, Mizumoto, Y, Kaziro
openaire +2 more sources
Viral RNA capping: Mechanisms and antiviral therapy
Journal of Medical VirologyAbstractRNA capping is an essential trigger for protein translation in eukaryotic cells. Many viruses have evolved various strategies for initiating the translation of viral genes and generating progeny virions in infected cells via synthesizing cap structure or stealing the RNA cap from nascent host messenger ribonucleotide acid (mRNA). In addition to
Saini Chen +5 more
openaire +2 more sources
The uncharted territory of NAD+-capped RNA
Trends in Cell BiologyThe critical redox cofactor NAD+ was recently reported to serve as an RNA cap in both eukaryotes and prokaryotes. However, its reversible regulation and biological functions remain unclear. Here, we provide insights into its discovery, capping and decapping mechanisms, for further discovery of their potential functional implications.
Quan Ma +3 more
openaire +2 more sources
Self-Capping RNA Catalysts Derived From Selection-Amplification
The Biological Bulletin, 1999The reactions of nucleoside 5’ triphosphates are among the most widespread (and therefore ancient) of the reactions of modern biochemistry. To study RNA enzymes that catalyze an important class of such reactions, we devised a new selection for RNAs that labilize their /3-y phosphates.
F, Huang, Z, Yang, M, Yarus
openaire +2 more sources