Paramyxovirus V proteins interact with the RNA helicase LGP2 to inhibit RIG-I-dependent interferon induction [PDF]
, 2012 This work was supported by the Wellcome Trust (grant AL087751/B)RIG-I and mda-5 are activated by viral RNA and stimulate type I interferon production. Laboratory of genetics and physiology 2 (LGP2) shares homology with RIG-I and mda-5 but lacks the CARD ...
Childs, Kay +2 more
core +1 more source
Structure of frequency-interacting RNA helicase from Neurospora crassa reveals high flexibility in a domain critical for circadian rhythm and RNA surveillance. [PDF]
PLoS ONE, 2018 The FRH (frequency-interacting RNA helicase) protein is the Neurospora crassa homolog of yeast Mtr4, an essential RNA helicase that plays a central role in RNA metabolism as an activator of the nuclear RNA exosome. FRH is also a required component of the
Yalemi Morales +3 more
doaj +1 more source
USP8‐Governed MDA5 Homeostasis Promotes Innate Immunity and Autoimmunity
Advanced Science, EarlyView.This study reveals that USP8 stabilizes MDA5 via AKT‐mediated phosphorylation (Ser718), enhancing their interaction and MDA5 deubiquitination. USP8 inactivation degrades MDA5, suppressing type I IFN and cytokine production. Pharmacological inhibition of USP8/AKT alleviates MDA5‐driven autoimmunity, demonstrating the USP8‐MDA5 axis as a therapeutic ...
Qimin Zhang +9 more
wiley +1 more source
Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]
, 2017 DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Klostermeier, Dagmar +5 more
core
Pif1 Helicase Lengthens Some Okazaki Fragment Flaps Necessitating Dna2 Nuclease/Helicase Action in the Two-nuclease Processing Pathway [PDF]
, 2009 We have developed a system to reconstitute all of the proposed steps of Okazaki fragment processing using purified yeast proteins and model substrates.
Bambara, Robert A. +3 more
core +2 more sources
Unwinding and Rewinding: Double Faces of Helicase?
Journal of Nucleic Acids, 2012 Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity—in other words, they can anneal two complementary single-stranded ...
Yuliang Wu
doaj +1 more source
Advanced Science, EarlyView.Akkermansia depletion correlates with acute pancreatitis (AP) severity. A. muciniphila and its protein Amuc_1409 mitigate pancreatic/systemic inflammation by expanding Tregs and IL‐10. Mechanistically, Amuc_1409 binds Ube2k, inhibiting Foxp3 ubiquitination to stabilize Treg differentiation and IL‐10 production, countering SAP‐induced SIRS/CARS ...
Jinyan Xie +9 more
wiley +1 more source
Flap Endonuclease Disengages Dna2 Helicase/Nuclease from Okazaki Fragment Flaps [PDF]
, 2006 Okazaki fragments contain an initiator RNA/DNA primer that must be removed before the fragments are joined. In eukaryotes, the primer region is raised into a flap by the strand displacement activity of DNA polymerase {delta}.
Bambara, Robert A. +2 more
core +1 more source
Helicase: A genetic tool for providing stress tolerance in plants
Plant Stress, 2023 Alteration of stress (abiotic and biotic) constitutes one of the major problems for normal plants health. In the present scenario, new molecular tools are broadly utilized to improve stress tolerance and high-yielding crop plants.
Monalisha Das Mohapatra +3 more
doaj
RNA helicase A is not required for RISC activity
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2013 It has been shown that siRNAs can compete with each other or with endogenous miRNAs for RISC components. This competition may complicate the interpretations of phenotypes observed through siRNA-mediated knockdown of genes, especially those genes implicated in the RISC pathway.
Xue-hai Liang, Stanley T. Crooke
openaire +3 more sources