Results 101 to 110 of about 342,007 (314)

Zika virus NS3 is a canonical RNA helicase stimulated by NS5 RNA polymerase [PDF]

Nucleic Acids Research, 2019
Abstract Zika virus is a positive single-strand RNA virus whose replication involved RNA unwinding and synthesis. ZIKV NS3 contains a helicase domain, but its enzymatic activity is not fully characterized. Here, we established a dsRNA unwinding assay based on the FRET effect to study the helicase activity of ZIKV NS3, which provided ...
Xu, Shan, Ci, Yali, Wang, Leijie, Yang, Yang, Zhang, Leiliang, Xu, Caimin, Qin, Chengfeng, Shi, Lei   +7 more
openaire   +2 more sources

Ketogenic Diet as an Epigenetic Therapy in SETD1B‐Related Epilepsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Histone lysine methyltransferases such as SETD1B regulate chromatin structure and gene transcription. Ketone bodies, including butyrate, act as histone deacetylase inhibitors. We report a 4‐year‐old boy with SETD1B‐related absence epilepsy, refractory to conventional medications, who achieved sustained > 90% seizure reduction on the Modified ...
Erica Tsang, Brian S. Gloss, Jessica P. Hayes, Andrew J. A. Holland, Manoj P. Menezes, Joceline A. Branson, Shekeeb S. Mohammad, Jingya J. Yan, Shrujna Patel, Velda X. Han, Russell C. Dale   +10 more
wiley   +1 more source

Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]

, 2010
As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates, Belousoff, Ben F. Luisi, Lilley, Marshall, Olorunniji, Rice, Richard P. Bowater, Sherratt, Smith, Szczelkun, Tomlinson, W. Marshall Stark, Weinzierl   +13 more
core   +1 more source

Selective Pharmacological Targeting of a DEAD Box RNA Helicase

PLoS ONE, 2008
RNA helicases represent a large family of proteins implicated in many biological processes including ribosome biogenesis, splicing, translation and mRNA degradation. However, these proteins have little substrate specificity, making inhibition of selected helicases a challenging problem.
Lindqvist, L, Oberer, M, Reibarkh, M, Cencic, R, Bordeleau, ME, Vogt, E, Marintchev, A, Tanaka, J, Fagotto, F, Altmann, M, Wagner, G, Pelletier, J   +11 more
openaire   +5 more sources

Unwinding and Rewinding: Double Faces of Helicase?

Journal of Nucleic Acids, 2012
Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity—in other words, they can anneal two complementary single-stranded ...
Yuliang Wu
doaj   +1 more source

Architecture of a SARS-CoV-2 mini replication and transcription complex

Nature Communications, 2020
SARS-CoV-2 virus replication and transcription is mediated by the replication and transcription complex (RTC) that is composed of 16 non-structural proteins (nsp). Here, the authors present the cryo-EM structure of a SARS-CoV-2 mini RTC consisting of the
Liming Yan, Ying Zhang, Ji Ge, Litao Zheng, Yan Gao, Tao Wang, Zhihui Jia, Haofeng Wang, Yucen Huang, Mingyu Li, Quan Wang, Zihe Rao, Zhiyong Lou   +12 more
doaj   +1 more source

Temporal and Cell‐Specific Regulation of Synaptic Homeostasis by the Chromatin Remodeler Chd1

Advanced Science, EarlyView.
Chd1, the Drosophila homologue of mammalian CHD2 ‐ a gene linked to autism, epilepsy, and intellectual disability, is required for synaptic homeostatic plasticity. Chd1 in glia is necessary for the rapid induction of synaptic homeostasis, whereas Chd1 in motoneurons, muscle, and glia is critical for long‐term maintenance.
Danielle T. Morency, Tao Cui, Yimei Cai, Chloe Lok, Rachel E. Nokku, Ruoxian Huang, Grace L. Chu, Yumeng Xie, Saleem W. Abu‐Tayeh, Kaikai He, Chengjie Qiu, Junyi Wang, Paxton M. Paganelli, Ting Wang, Gabrielle Williams, Sreejith Nair, Huadong Pei, Dion K. Dickman, Stefano Vicini, Tingting Wang   +19 more
wiley   +1 more source

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]

, 2017
DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Klostermeier, Dagmar, Linden, Martin H., Rudolph, Markus G., Steimer, Lenz, Wurm, Jan Philip, Wöhnert, Jens   +5 more
core  

RH70, a Bidirectional RNA Helicase, Co-purifies with U1snRNP [PDF]

Journal of Biological Chemistry, 2002
In this report, I describe the co-purification of a novel 70-kDa RNA helicase (RH70) and U1snRNP through six column steps. Peptide sequence analysis by mass spectrometry and Edman degradation revealed that RH70 is the previously reported DDX17. Biochemical characterization of RH70, obtained by partial separation from U1snRNP, yielded the following ...
openaire   +2 more sources

Structure of frequency-interacting RNA helicase from Neurospora crassa reveals high flexibility in a domain critical for circadian rhythm and RNA surveillance. [PDF]

PLoS ONE, 2018
The FRH (frequency-interacting RNA helicase) protein is the Neurospora crassa homolog of yeast Mtr4, an essential RNA helicase that plays a central role in RNA metabolism as an activator of the nuclear RNA exosome. FRH is also a required component of the
Yalemi Morales, Keith J Olsen, Jacqueline M Bulcher, Sean J Johnson   +3 more
doaj   +1 more source