Results 61 to 70 of about 92,282 (271)

RNA helicase A is not required for RISC activity

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2013
It has been shown that siRNAs can compete with each other or with endogenous miRNAs for RISC components. This competition may complicate the interpretations of phenotypes observed through siRNA-mediated knockdown of genes, especially those genes implicated in the RISC pathway.
Liang, Xue-hai, Crooke, Stanley T.
openaire   +2 more sources

Zika virus NS3 is a canonical RNA helicase stimulated by NS5 RNA polymerase [PDF]

Nucleic Acids Research, 2019
Abstract Zika virus is a positive single-strand RNA virus whose replication involved RNA unwinding and synthesis. ZIKV NS3 contains a helicase domain, but its enzymatic activity is not fully characterized. Here, we established a dsRNA unwinding assay based on the FRET effect to study the helicase activity of ZIKV NS3, which provided ...
Xu, Shan, Ci, Yali, Wang, Leijie, Yang, Yang, Zhang, Leiliang, Xu, Caimin, Qin, Chengfeng, Shi, Lei   +7 more
openaire   +2 more sources

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source

Unwinding and Rewinding: Double Faces of Helicase?

Journal of Nucleic Acids, 2012
Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity—in other words, they can anneal two complementary single-stranded ...
Yuliang Wu
doaj   +1 more source

Structure of frequency-interacting RNA helicase from Neurospora crassa reveals high flexibility in a domain critical for circadian rhythm and RNA surveillance. [PDF]

PLoS ONE, 2018
The FRH (frequency-interacting RNA helicase) protein is the Neurospora crassa homolog of yeast Mtr4, an essential RNA helicase that plays a central role in RNA metabolism as an activator of the nuclear RNA exosome. FRH is also a required component of the
Yalemi Morales, Keith J Olsen, Jacqueline M Bulcher, Sean J Johnson   +3 more
doaj   +1 more source

Analysis of the PcrA-RNA polymerase complex reveals a helicase interaction motif and a role for PcrA/UvrD helicase in the suppression of R-loops

eLife, 2021
The PcrA/UvrD helicase binds directly to RNA polymerase (RNAP) but the structural basis for this interaction and its functional significance have remained unclear.
Inigo Urrutia-Irazabal, James R Ault, Frank Sobott, Nigel J Savery, Mark S Dillingham   +4 more
doaj   +1 more source

Selective Pharmacological Targeting of a DEAD Box RNA Helicase

PLoS ONE, 2008
RNA helicases represent a large family of proteins implicated in many biological processes including ribosome biogenesis, splicing, translation and mRNA degradation. However, these proteins have little substrate specificity, making inhibition of selected helicases a challenging problem.
Lindqvist, L, Oberer, M, Reibarkh, M, Cencic, R, Bordeleau, ME, Vogt, E, Marintchev, A, Tanaka, J, Fagotto, F, Altmann, M, Wagner, G, Pelletier, J   +11 more
openaire   +5 more sources

Generation of an Induced Pluripotent Stem Cell‐Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments

Advanced Healthcare Materials, EarlyView.
Patient‐specific induced pluripotent stem cells (iPSCs) can be differentiated into alveolar type II cells (iAT2s), expanded as 3D alveolospheres, and grown at physiologically relevant air–liquid interface (ALI). This study shows for the first time the infectability of iAT2s by the influenza A virus (IAV) and proves their responsiveness to the well ...
Lena Gauthier, Hristina Koceva, Yann Bachelot, Rosanne W. Koutstaal, Puck B. van Kasteren, Marc Thilo Figge, Christian Eggeling, Alexander Sandy Mosig   +7 more
wiley   +1 more source

RH70, a Bidirectional RNA Helicase, Co-purifies with U1snRNP [PDF]

Journal of Biological Chemistry, 2002
In this report, I describe the co-purification of a novel 70-kDa RNA helicase (RH70) and U1snRNP through six column steps. Peptide sequence analysis by mass spectrometry and Edman degradation revealed that RH70 is the previously reported DDX17. Biochemical characterization of RH70, obtained by partial separation from U1snRNP, yielded the following ...
openaire   +2 more sources

EIF1AX Nucleolar Condensates Enhance Susceptibilities for the Management of Endometrial Cancer

Advanced Science, EarlyView.
This schematic illustrates the mechanism of a senolytic strategy in endometrial cancer. EIF1AX facilitates the incorporation of DDX21 into nucleolar condensates, an event that suppresses rDNA transcription and induces cellular senescence. The compound 2,5‐MeC exploits this pathway by promoting EIF1AX nucleolar translocation and condensate formation ...
Chengyu Lv, Zihang Lin, Jiandong Sun, Yuhong Ye, Qibin Wu, Liangzhi Cai, Dabin Liu, Pengming Sun, Shie Wang   +8 more
wiley   +1 more source