Results 131 to 140 of about 1,508,120 (294)

Impact of the Transboundary Interference Inhibitor on RNAi and the Baculovirus Expression System in Insect Cells

Insects
RNA interference inhibitors were initially discovered in plant viruses, representing a unique mechanism employed by these viruses to counteract host RNA interference.
Hao Zheng, Hengfeng Zhao, Haifan Xiong, Mian Muhammad Awais, Songrong Zeng, Jingchen Sun   +5 more
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Cosmic silence and viral noise: transcriptomic crosstalk in Caenorhabditis elegans under simulated space conditions

Frontiers in Microbiology
BackgroundSpaceflight environments pose unique physiological challenges due to al-tered gravity and radiation exposure.MethodsTo investigate how these abiotic stressors interact with viral infections, we ana-lyzed the transcriptomic response of ...
Ana Villena-Giménez, Esmeralda G. Legarda, Rubén González, Victoria G. Castiglioni, Victoria G. Castiglioni, Santiago F. Elena, Santiago F. Elena   +6 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

HIF-2α-dependent induction of miR-29a restrains TH1 activity during T cell dependent colitis

Nature Communications
Metabolic imbalance leading to inflammatory hypoxia and stabilization of hypoxia-inducible transcription factors (HIFs) is a hallmark of inflammatory bowel diseases.
Agnieszka K. Czopik, Eóin N. McNamee, Victoria Vaughn, Xiangsheng Huang, In Hyuk Bang, Trent Clark, Yanyu Wang, Wei Ruan, Tom Nguyen, Joanne C. Masterson, Eunyoung Tak, Sandra Frank, Colm B. Collins, Howard Li, Cristian Rodriguez-Aguayo, Gabriel Lopez-Berestein, Mark E. Gerich, Glenn T. Furuta, Xiaoyi Yuan, Anil K. Sood, Edwin F. de Zoeten, Holger K. Eltzschig   +21 more
doaj   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

RNA interference-based nanosystems for inflammatory bowel disease therapy

, 2016
Jian Guo,1 Xiaojing Jiang,1 Shuangying Gui1,2 1Department of Pharmaceutics, College of Pharmacy, Anhui University of Chinese Medicine, 2Institute of Pharmaceutics, Anhui Academy of Chinese Medicine, Hefei, Anhui, People’s Republic of China ...
Gui SY, Guo J, Jiang XJ
core  

Conditional RNA interference in mammalian cells via RNA transactivation

Nature Communications
RNA interference (RNAi) is a powerful tool for sequence-specific gene knockdown in therapeutic and research applications. However, spatiotemporal control of RNAi is required to decrease nonspecific targeting, potential toxicity, and allow targeting of ...
Yu Zhou, Peike Sheng, Jiayi Li, Yudan Li, Mingyi Xie, Alexander A. Green   +5 more
doaj   +1 more source

Reduced gravity and muon flux absence affect Caenorhabditis elegans life history traits and viral infection

Microbiology Spectrum
Environmental conditions fundamentally shape host-pathogen interactions; however, how multiple extreme abiotic stressors combine to influence infection outcomes remains poorly understood.
Ana Villena-Giménez, Victoria G. Castiglioni, Juan C. Muñoz-Sánchez, Esmeralda G. Legarda, Rubén González, Santiago F. Elena   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source