Selective Packaging in Murine Coronavirus Promotes Virulence by Limiting Type I Interferon Responses
mBio, 2018 Selective packaging is a mechanism used by multiple virus families to specifically incorporate genomic RNA (gRNA) into virions and exclude other types of RNA. Lineage A betacoronaviruses incorporate a 95-bp stem-loop structure, the packaging signal (PS),
Jeremiah Athmer +11 more
doaj +1 more source
Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes [PDF]
, 2007 The different steps of gene expression are intimately linked to coordinate and regulate this complex process. During transcription, numerous RNA-binding proteins are already loaded onto the nascent mRNA and package the mRNA into a messenger ...
Andersen, Ulrik L. +8 more
core +1 more source
Cooperation of an RNA Packaging Signal and a Viral Envelope Protein in Coronavirus RNA Packaging [PDF]
Journal of Virology, 2001 ABSTRACT Murine coronavirus mouse hepatitis virus (MHV) produces a genome-length mRNA, mRNA 1, and six or seven species of subgenomic mRNAs in infected cells. Among these mRNAs, only mRNA 1 is efficiently packaged into MHV particles. MHV N protein binds to all MHV mRNAs, whereas envelope M protein interacts only with mRNA 1. This
K, Narayanan, S, Makino
openaire +2 more sources
Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging. [PDF]
PLoS ONE, 2010 Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding
Yun Mai, Guangxia Gao
doaj +1 more source
The effect of RNA stiffness on the self-assembly of virus particles [PDF]
, 2017 Under many in vitro conditions, some small viruses spontaneously encapsidate a single stranded (ss) RNA into a protein shell called the capsid. While viral RNAs are found to be compact and highly branched because of long distance base-pairing between ...
Erdemci-Tandogan, Gonca +3 more
core +3 more sources
Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging [PDF]
Biochemical and Biophysical Research Communications, 2008 The bacteriophage phi29 DNA packaging motor is a protein/RNA complex that can produce strong force to condense the linear-double-stranded DNA genome into a pre-formed protein capsid. The RNA component, called the packaging RNA (pRNA), utilizes magnesium-dependent inter-molecular base-pairing interactions to form ring-shaped complexes.
Yun, Fang +4 more
openaire +2 more sources
Rotavirus Genomic RNA Complex Forms via Specific RNA-RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity. [PDF]
, 2017 Rotavirus (RV), a member of the Reoviridae family, causes infection in children and infants, with high morbidity and mortality. To be viable, the virus particle must package a set of eleven RNA segments.
Celma, Cristina C +3 more
core +1 more source
Alphavirus Nucleocapsid Packaging and Assembly
Viruses, 2018 Alphavirus nucleocapsids are assembled in the cytoplasm of infected cells from 240 copies of the capsid protein and the approximately 11 kb positive strand genomic RNA.
Adriano Mendes, Richard J. Kuhn
doaj +1 more source
NMR Studies of Retroviral Genome Packaging
Viruses, 2020 Nearly all retroviruses selectively package two copies of their unspliced RNA genomes from a cellular milieu that contains a substantial excess of non-viral and spliced viral RNAs.
Patricia S. Boyd +12 more
doaj +1 more source
Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
core +1 more source