Results 21 to 30 of about 113,079 (105)
Frontiers in Cellular and Infection Microbiology, 2023 Rift Valley fever virus (RVFV), a bunyavirus, has a single-stranded, negative-sense tri-segmented RNA genome, consisting of L, M and S RNAs. An infectious virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes ...
Breanna Tercero +8 more
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Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency
Molecular Therapy: Methods & Clinical Development, 2020 Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging ...
Eirini Vamva +3 more
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Selective Packaging in Murine Coronavirus Promotes Virulence by Limiting Type I Interferon Responses
mBio, 2018 Selective packaging is a mechanism used by multiple virus families to specifically incorporate genomic RNA (gRNA) into virions and exclude other types of RNA. Lineage A betacoronaviruses incorporate a 95-bp stem-loop structure, the packaging signal (PS),
Jeremiah Athmer +11 more
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Microbiology Spectrum, 2022 HIV-1 must package its RNA genome to generate infectious viruses. Recent studies have revealed that during genome packaging, HIV-1 not only excludes cellular mRNAs, but also distinguishes among full-length viral RNAs. Using NL4-3 and MAL molecular clones,
Jonathan M. O. Rawson +5 more
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Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging. [PDF]
PLoS ONE, 2010 Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding
Yun Mai, Guangxia Gao
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NMR Studies of Retroviral Genome Packaging
Viruses, 2020 Nearly all retroviruses selectively package two copies of their unspliced RNA genomes from a cellular milieu that contains a substantial excess of non-viral and spliced viral RNAs.
Patricia S. Boyd +12 more
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Alphavirus Nucleocapsid Packaging and Assembly
Viruses, 2018 Alphavirus nucleocapsids are assembled in the cytoplasm of infected cells from 240 copies of the capsid protein and the approximately 11 kb positive strand genomic RNA.
Adriano Mendes, Richard J. Kuhn
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Retrovirology, 2007 Background Retroviruses selectively encapsidate two copies of their genomic RNA, the Gag protein binding a specific RNA motif in the 5' UTR of the genome. In human immunodeficiency virus type 2 (HIV-2), the principal packaging signal (Psi) is upstream of
Crowther R Anthony +3 more
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Dimeric RNA recognition regulates HIV-1 genome packaging.
PLoS Pathogens, 2013 How retroviruses regulate the amount of RNA genome packaged into each virion has remained a long-standing question. Our previous study showed that most HIV-1 particles contain two copies of viral RNA, indicating that the number of genomes packaged is ...
Olga A Nikolaitchik +9 more
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MoMuLV and HIV-1 nucleocapsid proteins have a common role in genomic RNA packaging but different in late reverse transcription. [PDF]
PLoS ONE, 2012 Retroviral nucleocapsid proteins harbor nucleic acid chaperoning activities that mostly rely on the N-terminal basic residues and the CCHC zinc finger motif.
Célia Chamontin +4 more
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