From Cells to Virus Particles: Quantitative Methods to Monitor RNA Packaging [PDF]
Viruses, 2016 In cells, positive strand RNA viruses, such as Retroviridae, must selectively recognize their full-length RNA genome among abundant cellular RNAs to assemble and release particles.
Mireia Ferrer +4 more
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Human Retrovirus Genomic RNA Packaging [PDF]
Viruses, 2022 Two non-covalently linked copies of the retrovirus genome are specifically recruited to the site of virus particle assembly and packaged into released particles.
Heather M. Hanson +3 more
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RNA Structures and Their Role in Selective Genome Packaging
Viruses, 2021 To generate infectious viral particles, viruses must specifically select their genomic RNA from milieu that contains a complex mixture of cellular or non-genomic viral RNAs.
Liqing Ye +7 more
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The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. [PDF]
PLoS Computational Biology, 2021 The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the ...
Sam R Hill +2 more
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Packaging of Genomic RNA in Positive-Sense Single-Stranded RNA Viruses: A Complex Story
Viruses, 2019 The packaging of genomic RNA in positive-sense single-stranded RNA viruses is a key part of the viral infectious cycle, yet this step is not fully understood. Unlike double-stranded DNA and RNA viruses, this process is coupled with nucleocapsid assembly.
Mauricio Comas-Garcia
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MMTV RNA packaging requires an extended long-range interaction for productive Gag binding to packaging signals. [PDF]
PLoS BiologyThe packaging of genomic RNA (gRNA) into retroviral particles relies on the specific recognition by the Gag precursor of packaging signals (Psi), which maintain a complex secondary structure through long-range interactions (LRIs).
Suresha G Prabhu +8 more
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Kinetic Studies on the Interaction of HIV-1 Gag Protein with the HIV-1 RNA Packaging Signal [PDF]
VirusesDuring HIV-1 virus assembly, the genomic RNA (vRNA) is selected for packaging by the viral protein Gag because it contains a specific packaging signal, Psi. While there have been numerous studies of Gag–Psi interactions, there is almost no information on
Constance Rink +3 more
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Nucleocapsid protein-dependent assembly of the RNA packaging signal of Middle East respiratory syndrome coronavirus [PDF]
Journal of Biomedical Science, 2018 Background Middle East respiratory syndrome coronavirus (MERS-CoV) consists of a positive-sense, single-stranded RNA genome and four structural proteins: the spike, envelope, membrane, and nucleocapsid protein. The assembly of the viral genome into virus
Wei-Chen Hsin +6 more
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Identification of the SARS-CoV-2 genome packaging signal in the nsp12-coding region [PDF]
Nature CommunicationsSelective genome packaging is a critical step for RNA viruses, which must distinguish genomic RNA from other abundant transcripts. For SARS-CoV-2, the cis-acting packaging signal is thought to be recognized by the nucleocapsid (N) protein, but its ...
Youngran Park +6 more
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Involvement of 5′ and 3′ UTRs in SARS-CoV-2 Virus-like Particle Genome Packaging [PDF]
VirusesThe molecular mechanisms governing the efficient packaging of the large SARS-CoV-2 RNA genome into progeny virions remain incompletely understood, with the role of untranslated regions (UTRs) being particularly enigmatic.
Zhang Zhang +12 more
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