Structural requirements for efficient translational frameshifting in the synthesis of the putative viral RNA-dependent RNA polymerase of potato leafroll virus [PDF]
, 1993 Alicja B. Kujawa +3 more
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Azotobacter vinelandii RNA polymerase. II - Effect of ribonuclease on polymerase activity [PDF]
Ribonuclease effect on polymerase activity during ribonucleic acid synthesis ...
Krakow, J. S.
core +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Ordered assembly of a functional preinitiation transcription complex, containing vaccinia virus early transcription factor and RNA polymerase, on an immobilized template [PDF]
, 1994 Carl J. Baldick +3 more
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Developing an Analytical Method for Separating and Quantifying RNA Generated in In Vitro Transcription Reactions [PDF]
, 2015 The generation of run-off transcripts from in vitro transcription reactions is a useful technique in the study of transcription regulation. There are currently limited ways in which these run-off transcripts can be analyzed, and few that are truly ...
Wienkers, Henry
core +1 more source
Template-directed biopolymerization: tape-copying Turing machines
, 2012 DNA, RNA and proteins are among the most important macromolecules in a living cell. These molecules are polymerized by molecular machines. These natural nano-machines polymerize such macromolecules, adding one monomer at a time, using another linear ...
Chowdhury, Debashish, Sharma, Ajeet K.
core +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Molecular Dynamics Simulation Trajectories of the Taq RNA Polymerase Holoenzyme
, 2021 Ilona Christy Unarta
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Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe
FEBS Open Bio, EarlyView.Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley +1 more source
RNA Polymerase II CTD Tyrosine 1 Is Required for Efficient Termination by the Nrd1-Nab3-Sen1 Pathway [PDF]
, 2019 Pierre Collin +3 more
openalex +1 more source