Results 351 to 360 of about 2,633,578 (383)
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Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
Science, 1992Effective methods are needed to identify and isolate those genes that are differentially expressed in various cells or under altered conditions. This report describes a method to separate and clone individual messenger RNAs (mRNAs) by means of the ...
Peng Liang, A. Pardee
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Critical Reviews in Biochemistry, 1988
Recent progress in molecular biological techniques revealed that genomes of animal viruses are complex in structure, for example, with respect to the chemical nature (DNA or RNA), strandedness (double or single), genetic sense (positive or negative), circularity (circle or linear), and so on.
Akira Ishihama, Kyosuke Nagata
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Recent progress in molecular biological techniques revealed that genomes of animal viruses are complex in structure, for example, with respect to the chemical nature (DNA or RNA), strandedness (double or single), genetic sense (positive or negative), circularity (circle or linear), and so on.
Akira Ishihama, Kyosuke Nagata
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Molecular Biology, 2005
Plastids have a very interesting transcription apparatus that gives us an opportunity to investigate mono- and multisubunut RNA polymerase interaction under conditions of complex biogenesis of the organelles and the necessity to coordinate the expression of genes located in different cell compartments.
Vladimir V. Kuznetsov, E. A. Lysenko
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Plastids have a very interesting transcription apparatus that gives us an opportunity to investigate mono- and multisubunut RNA polymerase interaction under conditions of complex biogenesis of the organelles and the necessity to coordinate the expression of genes located in different cell compartments.
Vladimir V. Kuznetsov, E. A. Lysenko
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2003
Abstract Bacteriophage T7 RNA polymerase (RNAP) is the best characterized member of a widespread family of RNAPs that includes most bacteriophage-encoded RNAPs as well as the mitochondrial RNAPs. The robust activity and strict promoter specificity of the phage RNAPs have made them extremely useful as reagents for both in vitro preparation of ...
Srabani Mukherjee, Rui J Sousa
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Abstract Bacteriophage T7 RNA polymerase (RNAP) is the best characterized member of a widespread family of RNAPs that includes most bacteriophage-encoded RNAPs as well as the mitochondrial RNAPs. The robust activity and strict promoter specificity of the phage RNAPs have made them extremely useful as reagents for both in vitro preparation of ...
Srabani Mukherjee, Rui J Sousa
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The genetics of RNA polymerases in yeasts [PDF]
Over the past few years, substantial progress has been made in the genetic characterization of yeast RNA polymerases. Most of their genes have been identified and their complete cloning and sequencing is within reach. Several conclusions can be drawn at this stage.
Mosrin-Huaman, Christine, Thuriaux, P
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Current Opinion in Structural Biology, 2001
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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Picornavirus RNA-dependent RNA polymerase
The International Journal of Biochemistry & Cell Biology, 2009Replication of the picornavirus genome is catalysed by a viral encoded RNA-dependent RNA polymerase, termed 3D polymerase. Together with other viral and host proteins, this enzyme performs its functions in the cytoplasm of host cells. The crystal structure of 3D polymerase from a number of picornaviruses has been determined.
Peter C. McMinn, Chee Choy Kok
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Ribosomal RNA Synthesis and RNA Polymerase
Nature, 1970MULTIPLE forms of RNA polymerase activity have been isolated from developing sea-urchin embryos, rat liver nuclei and calf thymus1–3. The molecular basis of this heterogeneity is at present unknown but, whatever its basis, the crucial question seems to be whether different enzymes copy different genes.
G. P. Tocchini-Valentini, M. Crippa
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2004
At the end of the 1950s Hoagland and Zamecnik had succeeded in obtaining an in vitro protein synthesis from ribosomes and crude acellular extracts. Ribosomes cannot by themselves achieve a specific protein synthesis and require an information originating in last analysis in the genes.
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At the end of the 1950s Hoagland and Zamecnik had succeeded in obtaining an in vitro protein synthesis from ribosomes and crude acellular extracts. Ribosomes cannot by themselves achieve a specific protein synthesis and require an information originating in last analysis in the genes.
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Journal of Molecular Biology, 2000
Bacterial RNA polymerase and eukaryotic RNA polymerase II exhibit striking structural similarities, including similarities in overall structure, relative positions of subunits, relative positions of functional determinants, and structures and folding topologies of subunits.
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Bacterial RNA polymerase and eukaryotic RNA polymerase II exhibit striking structural similarities, including similarities in overall structure, relative positions of subunits, relative positions of functional determinants, and structures and folding topologies of subunits.
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