alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis [PDF]
, 2004 At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the ...
Brannigan, J.A., Kumar, A., Moran, C.P.
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Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Science, 2018 Phase separation and gene control Many components of eukaryotic transcription machinery—such as transcription factors and cofactors including BRD4, subunits of the Mediator complex, and RNA polymerase II—contain intrinsically disordered low-complexity ...
W. Cho +6 more
semanticscholar +1 more source
RNA Polymerase-Promoter Interactions: the Comings and Goings of RNA Polymerase [PDF]
Journal of Bacteriology, 1998 Initiation of transcription is a complicated process involving several different phases: promoter location by RNA polymerase, formation of a competent initiation complex, synthesis of the initial phosphodiester bonds, and movement of RNA polymerase from the promoter as it enters the elongation ...
Margaret L. Zupancic +2 more
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Characterization of RNA polymerases from Rous sarcoma virus-induced mouse ascites sarcoma cells. [PDF]
, 1979 RNA polymerase was extracted from the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV)-induced C3H/He mouse ascites sarcoma cells (SR-C3H). RNA polymerase was separated into RNA polymerases I and II by DEAE-Sephadex chromatography.
Misumi, Hiromasa, Oda, Takuzo
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An RNA polymerase ribozyme that synthesizes its own ancestor
Proceedings of the National Academy of Sciences of the United States of America, 2020 Significance Life depends on the propagation of heritable information across successive generations. An RNA polymerase ribozyme was obtained by in vitro evolution that has an unprecedented level of activity in copying complex RNA templates.
Katrina F. Tjhung +3 more
semanticscholar +1 more source
N4 RNA Polymerase II, a Heterodimeric RNA Polymerase with Homology to the Single-Subunit Family of RNA Polymerases [PDF]
Journal of Bacteriology, 2002 ABSTRACTBacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3.
R. H. Carter +3 more
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The largest subunit of human RNA polymerase III is closely related to the largest subunit of yeast and trypanosome RNA polymerase III [PDF]
, 1997 In both yeast and mammalian systems, considerable progress has been made toward the characterization of the transcription factors required for transcription by RNA polymerase III. However, whereas in yeast all of the RNA polymerase III subunits have been
Hernandez, N., Sepehri, S.
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Biogenesis of RNA Polymerases in Yeast [PDF]
Frontiers in Molecular Biosciences, 2021 Eukaryotic RNA polymerases (RNA pols) transcriptional processes have been extensively investigated, and the structural analysis of eukaryotic RNA pols has been explored. However, the global assembly and biogenesis of these heteromultimeric complexes have been narrowly studied.
Ana I. Garrido-Godino +3 more
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A link between transcription fidelity and pausing in vivo [PDF]
, 2017 Pausing by RNA polymerase is a major mechanism that regulates transcription elongation but can cause conflicts with fellow RNA polymerases and other cellular machineries. Here, we summarize our recent finding that misincorporation could be a major source
Gamba, Pamela +2 more
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Syn5 RNA polymerase synthesizes precise run-off RNA products [PDF]
, 2013 The enzyme predominantly used for in vitro run-off RNA synthesis is bacteriophage T7 RNA polymerase. T7 RNA polymerase synthesizes, in addition to run-off products of precise length, transcripts with an additional non-base-paired nucleotide at the 3 ...
Richardson, Charles C. +2 more
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