Results 221 to 230 of about 587,888 (232)

Hybridization and detection of digoxigenin probes on RNA blots

Molecular Biotechnology, 1996
This article describes nonradioactive probing of a Northern blot. The method employs digoxigenin-labeled probes. Antidigoxigenin antibody/alkaline phosphate conjugate, and a chemiluminescent substrate are subsequently used in the detection system.
Elizabeth Davies, Rachel Hodge, Peter G. Isaac   +2 more
openaire   +5 more sources

Chemical Probing of RNA in Living Cells

2013
RNAs need to adopt a specific architecture to exert their task in cells. While significant progress has been made in describing RNA folding landscapes in vitro, understanding intracellular RNA structure formation is still in its infancy. This is in part due to the complex nature of the cellular environment but also to the limited availability of ...
Andreas Liebeg, Verena Heisig, Michael Wildauer, Christina Waldsich, Georgeta Zemora   +4 more
openaire   +3 more sources

Probing RNA Structure Within Living Cells

2009
RNA folding is the most fundamental process underlying RNA function. RNA structure and associated folding paradigms have been intensively studied in vitro. However, in vivo RNA structure formation has only been explored to a limited extent. To determine the influence of the cellular environment, which differs significantly from the in vitro refolding ...
Andreas Liebeg, Christina Waldsich
openaire   +3 more sources

Spectroscopic Probes of RNA Structure and Dynamics

2012
A single technique is insufficient to characterize the properties of an RNA molecule so this Guide provides advice and suggestions for use of several spectroscopic methods applied to RNA. It begins with a discussion of design features to synthesize a suitable molecule for study, assuming that the reader is familiar with in vitro enzymatic and chemical ...
openaire   +2 more sources

[1] Probing RNA structure and RNA-ligand complexes with chemical probes

2000
Christine Brunel, Pascale Romby
openaire   +3 more sources

In-gel fluorescence probing of RNA–RNA interactions

Analytical Biochemistry, 2007
Nadukkudy V. Eldho, Orlando H. Gumbs, Michalynn Thompson, Kwaku T. Dayie, Mahadevan Seetharaman   +4 more
openaire   +3 more sources

Probes in DNA/RNA Hybridization

Biologicals, 1996
openaire   +3 more sources

In situ Hybridization with RNA Probes

2003
openaire   +3 more sources

RNA Stable Isotope Probing

2014
Manefield, Mike, Gutierrez-Zamora, Maria-Luisa, Whiteley, Andrew S.   +2 more
openaire   +2 more sources

Widening the probe

Nature Chemical Biology, 2016
openaire   +3 more sources