Results 71 to 80 of about 587,888 (232)
BMC Biotechnology, 2007 Background In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g.
Hamilton-Dutoit Stephen +4 more
doaj +1 more source
Hybridization of RNA Probes v1
, 2020 Over the past decade a plethora of noncoding RNAs (ncRNAs) have been identified, initiating an explosion in RNA research. Although RNA sequencing methods provide unsurpassed insights into ncRNA distribution and expression, detailed information on structure and processing are harder to extract from sequence data.
Jonathan Houseley, Cristina Cruz
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Molecular Therapy: Nucleic Acids, 2022 We successfully fabricated threose nucleic acid (TNA)-based probes for real-time monitoring of target miRNA levels in cells. Our TNA probe is comprised of a fluorophore-labeled TNA reporter strand by partially hybridizing to a quencher-labeled TNA that ...
Fei Wang +5 more
doaj
Fluorescent Probes for Nucleic Acid Visualization in Fixed and Live Cells
Molecules, 2013 This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to
Alexandre S. Boutorine +4 more
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The Benzothiazine Core as a Novel Motif for DNA-Binding Small Molecules
Molecules, 2023 A new series of 4H-1,3-benzothiazine dyes were prepared and fully characterized in an aqueous medium. Benzothiazine salts were synthesized either through the classical synthetic pathway using Buchwald–Hartwig amination or through economical and ...
Milena Mlakić +10 more
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Probing complex RNA structures by mechanical force [PDF]
The European Physical Journal E, 2003 9 pages, 9 ...
Isambert H +7 more
openaire +6 more sources
Viruses, 2018 In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues.
Vanessa M. Pfankuche +14 more
doaj +1 more source
nextPARS: Parallel probing of RNA structures in Illumina [PDF]
RNA, 2017 ABSTRACTRNA molecules play important roles in virtually every cellular process. These functions are often mediated through the adoption of specific structures that enable RNAs to interact with other molecules. Thus, determining the secondary structures of RNAs is central to understanding their function and evolution.
Heinz Himmelbauer +9 more
openaire +5 more sources
Orchestrated transcription of biological processes in the marine picoeukaryote Ostreococcus exposed to light/dark cycles [PDF]
, 2010 Background: Picoeukaryotes represent an important, yet poorly characterized component of marine phytoplankton. The recent genome availability for two species of Ostreococcus and Micromonas has led to the emergence of picophytoplankton comparative ...
Bouget, François-Yves +7 more
core +7 more sources
Do-it-yourself: construction of a custom cDNA macroarray platform with high sensitivity and linear range [PDF]
, 2011 Background: Research involving gene expression profiling and clinical applications, such as diagnostics and prognostics, often require a DNA array platform that is flexibly customisable and cost-effective, but at the same time is highly sensitive and ...
Boonefaes, Tom +7 more
core +3 more sources