Results 81 to 90 of about 587,888 (232)

Error, reproducibility and sensitivity : a pipeline for data processing of Agilent oligonucleotide expression arrays [PDF]

, 2010
Background Expression microarrays are increasingly used to obtain large scale transcriptomic information on a wide range of biological samples. Nevertheless, there is still much debate on the best ways to process data, to design experiments and analyse ...
AR Dabney, AR Dabney, Benjamin Chain, BM Bolstad, BP Durbin, BS Everitt, CR Hampton, D Wang, E Birney, Helen Bowen, J Fan, J Rasaiyaah, J Rasaiyaah, Jane Rasaiyaah, Jhen Tsang, John Hammond, JP Hammond, L Shi, M Noursadeghi, M Sultan, Mahdad Noursadeghi, MN McCall, PA 't Hoen, TA Patterson, TC Kroll, WE Johnson, Wilfried Posch   +26 more
core   +2 more sources

Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II

BioTechniques, 2016
The high sequence variation of RNA viruses necessitates use of degenerate primers and probes or multiple primers and probes in molecular diagnostic assays. We showed previously that PCR amplification in two rounds, first with long target-specific primers
Hongyan Xia, Sabine Gravelsina, Christina Öhrmalm, Jakob Ottoson, Jonas Blomberg   +4 more
doaj   +1 more source

Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]

F1000Research, 2016
Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic ...
Lilith V.J.C. Mannack, Sebastian Eising, Andrea Rentmeister   +2 more
doaj   +1 more source

Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens

Microsystems & Nanoengineering, 2023
To minimize and control the transmission of infectious diseases, a sensitive, accurate, rapid, and robust assay strategy for application on-site screening is critical. Here, we report single-molecule RNA capture-assisted digital RT-LAMP (SCADL) for point-
Liying Jiang, Xianghao Lan, Linjiao Ren, Zhiyuan Jin, Xuchen Shan, Mingzhu Yang, Lingqian Chang   +6 more
doaj   +1 more source

Quantitative single-cell splicing analysis reveals an ‘economy of scale’ filter for gene expression [PDF]

, 2018
In eukaryotic cells, splicing affects the fate of each pre-mRNA transcript, helping to determine whether it is ultimately processed into an mRNA, or degraded. The efficiency of splicing plays a key role in gene expression.
Ding, Fangyuan, Elowitz, Michael B.
core  

Differential regulation of expression of the multiple ADP/ATP translocase genes in human cells [PDF]

, 1991
The expression of the genes encoding the three isoforms of the human ADP/ATP translocase (T1, T2, and T3) has been investigated in cultured cell systems under different experimental conditions, using isoform- specific probes.
Attardi, Giuseppe, Lunardi, Joël
core  

Real-time DNA microarray analysis [PDF]

, 2009
We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e.
Abdueva, Arjang Hassibi, Babak Hassibi, Bassil, Beissbarth, Belosludtsev, Berg, Bishop, Chee, Chen, Dai, Das, Fritz, Georgiadis, Ghosh, Hall, Hardenbol, Haris Vikalo, Hassibi, Hassibi, Hassibi, Heaton, Homola, Hubble, Huber, José Luis Riechmann, Kennedy, Lacowicz, Levine, Linneti, Lockhart, Lockhart, Marras, Okamura, Ota, Pease, Peterson, Rajendran, SantaLucia, Savran, Schena, Schena, Schienle, Shalon, Skvortsov, Smith, Steinfield, Stimpson, Su, Towery, Tu, Van Kampen, Vikalo, Wang, Watterson, Yamaguchi, Zeng   +56 more
core   +3 more sources

Protein-mediated miRNA detection and siRNA enrichment using p19

BioTechniques, 2010
p19 RNA binding protein from the Carnation Italian ringspot virus (CIRV) is an RNA-silencing suppressor that binds small interfering RNA (siRNA) with high affinity.
Jingmin Jin, Melissa Cid, Catherine B. Poole, Larry A. McReynolds   +3 more
doaj   +1 more source

Exploring RNA-targeted gene therapy approaches for hypertrophic cardiomyopathy [PDF]

, 2012
Relatório de projeto no âmbito do Programa de Bolsas Universidade de Lisboa/Fundação Amadeu Dias (2011/2012). Universidade de Lisboa.
Freitas, Ana
core  

A Hox gene mutation that triggers Nonsense-mediated RNA decay and affects alternative splicing during Drosophila development [PDF]

, 2003
Nonsense mutations are usually assumed to affect protein function by generating truncated protein products. Nonetheless, it is now clear that these mutations affect not just protein synthesis but also messenger RNA stability.
Akam, Michael, Alonso, Claudio R
core   +2 more sources