Results 81 to 90 of about 616,337 (352)

psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation

Nature Communications, 2021
ATP-dependent helicases remodel the spliceosome and proofread splice site recognition. A new method – Purified Spliceosome iCLIP (psiCLIP) – probes protein-RNA interactions in defined spliceosome complexes to reveal how the helicases Prp16 and Prp22 ...
Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule, Kiyoshi Nagai   +9 more
doaj   +1 more source

Effective affinities in microarray data

, 2006
In the past couple of years several studies have shown that hybridization in Affymetrix DNA microarrays can be rather well understood on the basis of simple models of physical chemistry.
, Alberts B, Bloomfield V A, Burden C J Pittelkow Y Wilson S R, Carlon E, Carlon E Heim T, Deutsch J M Liang S Narayan O, E Carlon, Forman J E, G T Barkema, Halperin A, J Klein Wolterink, Lipshutz R J, Lyklema J, T Heim   +14 more
core   +1 more source

DNA hybridization to mismatched templates: a chip study [PDF]

, 2001
High-density oligonucleotide arrays are among the most rapidly expanding technologies in biology today. In the {\sl GeneChip} system, the reconstruction of the target concentration depends upon the differential signal generated from hybridizing the ...
A. Bonincontro, C. Li, D. Cule, D.K. Lubensky, Daniel A. Lim, Felix Naef, G. Bonnet, G. Vesnaver, J. Lockhart, J.A.D. Wattis, M. Chee, M. Peyrard, M. Salerno, Marcelo Magnasco, N. Singh, N.L. Goddard, Nila Patil, R.J. Lipshutz, Y. Zhang, Z. Haijun   +19 more
core   +2 more sources

Probing RNA Structure by Lead Cleavage [PDF]

Current Protocols in Nucleic Acid Chemistry, 2000
AbstractLead cleavage causes a transesterification reaction that breaks the 5′,3′‐phosphodiester backbone of RNA, leaving a 2′,3′‐cyclic phosphate and a 5′‐hydroxyl. Since the efficiency of the reaction at the 2′‐hydroxyl is related to steric and chemical constraints on particular 2′‐hydroxyls embedded in the RNA, this reaction can be used to examine ...
openaire   +3 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

HybriSeq: probe-based device-free single-cell RNA profiling

Communications Biology
We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can
Daniel Foyt, David Brown, Shuqin Zhou, Brittany Moser, Qin Zhu, Zev J. Gartner, Bo Huang   +6 more
doaj   +1 more source

Fluorescent Probes for Nucleic Acid Visualization in Fixed and Live Cells

Molecules, 2013
This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to
Alexandre S. Boutorine, Darya S. Novopashina, Olga A. Krasheninina, Karine Nozeret, Alya G. Venyaminova   +4 more
doaj   +1 more source

Gene Expression and the Physiological Role of Transforming Growth Factor-α in the Mouse Pituitary [PDF]

, 2003
Transforming growth factor-alpha (TGF-alpha), a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-alpha-expressing cells and the physiological roles of TGF-a within mouse
Kakeya, Tomoshi, Masui, Tohru, Oomizu, Souichi, Sharma, Seema, Takahashi, Sumio, Takeuchi, Sakae   +5 more
core   +1 more source

Probing the phenomics of noncoding RNA [PDF]

eLife, 2013
Genetic knockout experiments on mice confirm that some long noncoding RNA molecules have developmental functions.
openaire   +3 more sources

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source