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RNA SEQUENCE ANALYSIS

Annual Review of Biochemistry, 1970
FRACTIONATION AND PURIFICATION OF RNA 228 Transfer RNA 228 Ribosomal RNA ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 230 Viral RNA 230 SEPARATION AND ISOLATION OF POLYNUCLEOTIDES 231 Physical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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RNA sequencing and Prediction Tools for Circular RNAs Analysis

2018
Circular RNAs (circRNAs) are noncoding and single-stranded RNA transcripts able to form covalently circular-closed structures. They are generated through alternative splicing events and widely expressed from human to viruses. CircRNAs have been appointed as potential regulators of microRNAs (miRNAs), RNA-binding proteins (RPBs), and lineal protein ...
Elena, López-Jiménez   +2 more
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In Silico Analysis of Micro-RNA Sequencing Data

2021
High-throughput sequencing for micro-RNAs (miRNAs) to obtain expression estimates is a central method of molecular biology. Surprisingly, there are a number of different approaches to converting sequencing output into micro-RNA counts. Each has their own strengths and biases that impact on the final data that can be obtained from a sequencing run. This
Ernesto, Aparicio-Puerta   +3 more
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Sequence and Structure Analysis of Noncoding RNAs

2009
Noncoding RNAs (ncRNAs) are increasingly recognized as important functional molecules in the cell. Here we give a short overview of fundamental computational techniques to analyze ncRNAs that can help us better understand their function. Topics covered include prediction of secondary structure from the primary sequence, prediction of consensus ...
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Analysis of Pteridium ribosomal RNA sequences by rapid direct sequencing

Plant Molecular Biology, 1991
A total of 864 bases from 5 regions interspersed in the 18S and 26S rRNA molecules from various clones of Pteridium covering the general geographical distribution of the genus was analysed using a rapid rRNA sequencing technique. No base difference has been detected amongst the three major lineages, two of which apparently separated before the breakup ...
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Differential analysis of rna methylation sequencing data

2013 IEEE Global Conference on Signal and Information Processing, 2013
Recently, a new technique, combining Methylated RNA Immunoprecipatation with RNA sequencing (MeRIP-Seq), was developed and applied to survey the global mRNA N6-methyladenosine (m6A) methylation in mammalian cells. MeRIP-Seq has the potential to survey, for the first time, the transcriptome-wide distribution of different types of post-transcriptional ...
Xiaodong Cui   +4 more
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RNA Sequences Similarities Analysis by Inner Products

2015
According to 2-D chaos game representation of RNA secondary structures, we propose a new method of 3-D graphical representation which does not lose any biological information. Then we extract a new numerical feature which called inner products from 3-D graphical representation. It is regarded as a value to analyze the relationship between nine kinds of
Shanshan Xing   +3 more
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Massively Parallel Analysis of Regulatory RNA Sequences

2021
The stability of RNA transcripts is regulated by signals within their sequences, but the identity of those signals still remain elusive in many biological systems. Recently introduced massively parallel tools for the analysis of regulatory RNA sequences provide the ability to detect functional cis-regulatory sequences of post-transcriptional RNA ...
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Bioinformatic Analysis of Small RNA Sequencing Libraries

2019
Bioinformatic analysis of small RNA sequencing libraries consists of transforming a series of small RNA sequencing experiment fastq files into a table containing small RNA sequences and their abundance. This is achieved by cleaning the reads, aligning the cleaned reads to a reference, and parsing the alignment results.
Ricardo A, Chávez Montes   +2 more
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Vaccinia Virus Transcriptome Analysis by RNA Sequencing

2019
RNA-sequencing (RNA-Seq) using next-generation sequencing (NGS) technique is a powerful tool for simultaneous analysis of global transcripts from both vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells.
Shuai, Cao, Yongquan, Lin, Zhilong, Yang
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