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RNA-Sequencing of Staphylococcus aureus Messenger RNA
2014RNA-sequencing (RNA-seq) is a technique that employs next-generation DNA-sequencing technology to simultaneously sequence all of the RNA transcripts in a cell. It can provide valuable insights into transcript and operon structure, and is rapidly replacing expression microarrays as the technique of choice for determining global gene expression profiles ...
Ronan K, Carroll +2 more
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Maternal plasma RNA sequencing
Clinical Biochemistry, 2015This review discusses the question: which steps are essential or need to be optimized so that maternal plasma RNA sequencing for pre-eclampsia and related syndromes will become as specific and sensitive as maternal plasma DNA sequencing for trisomy?
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Normalized Similarity of RNA Sequences
2005We introduce a normalized version of the LCS metric as a new local similarity measure for comparing two RNAs. An $\mathcal{O}(n^{2}m{\rm lg}m)$ time algorithm is presented for computing the maximum normalized score of two RNA sequences, where n and m are the lengths of the sequences and n ≤ m.
Rolf Backofen +3 more
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RNA Sequencing Protocols for Short-Read Sequencing
RNA sequencing (RNA-seq) methodologies allow the discovery of novel variants and transcripts. These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform.Laura, Vasquez-Velez +2 more
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Terminal Sequences of Bacteriophage RNAs
Nature, 1968The 3′OH terminal oligonucleotides of f2, R17 and QβRNA, isolated by two-dimensional diagonal electrophoresis, have been sequenced. In addition, nucleotide triphosphates have been identified at the 5′ end of f2 and QβRNA.
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Nuclear RNA Isolation and Sequencing
2016Most transcriptome studies involve sequencing and quantification of steady-state mRNA by isolating and sequencing poly (A) RNA. Although this type of sequencing data is informative to determine steady-state mRNA levels it does not provide information on transcriptional output and thus may not always reflect changes in transcriptional regulation of gene
Navroop K, Dhaliwal +1 more
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RNA Sequencing by Primer Extension
Cold Spring Harbor Protocols, 2013Direct sequencing of RNA by primer extension is a fast and accurate method that is useful in a variety of situations. It is a valuable technique for determining the faithfulness of in vitro processing reactions, such as splicing or RNA editing. It is often used as an alternative to reverse transcription PCR (RT–PCR) followed by cloning and DNA ...
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Circular RNAs sequenced at last
Nature Biotechnology, 2021Zhao Zhang, Leng Han
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