Results 261 to 270 of about 1,474,432 (298)

RNA-Sequencing of Staphylococcus aureus Messenger RNA

2014
RNA-sequencing (RNA-seq) is a technique that employs next-generation DNA-sequencing technology to simultaneously sequence all of the RNA transcripts in a cell. It can provide valuable insights into transcript and operon structure, and is rapidly replacing expression microarrays as the technique of choice for determining global gene expression profiles ...
Ronan K, Carroll, Andy, Weiss, Lindsey N, Shaw   +2 more
openaire   +2 more sources

Nanopore RNA Sequencing Analysis

2021
The recent advent of Nanopore sequencing allows for the sequencing of full-length RNA or cDNA molecules. This new type of data introduces new challenges from the computational point of view, and requires new software as well as dedicated analysis pipelines.
Tommaso, Leonardi, Adrien, Leger
openaire   +2 more sources

Sequencing diverse RNA modifications

Nature Methods, 2021
Nanopore sequencing enables quantitative profiling of pseudouridine and 2′-O-methylation modifications in native RNAs.
openaire   +2 more sources

Maternal plasma RNA sequencing

Clinical Biochemistry, 2015
This review discusses the question: which steps are essential or need to be optimized so that maternal plasma RNA sequencing for pre-eclampsia and related syndromes will become as specific and sensitive as maternal plasma DNA sequencing for trisomy?
openaire   +2 more sources

RNA sequencing

1990
Abstract The first complete nucleotide sequence of a gene was published in 1964 by Robert Holley and associates. (1). This sequence was inferred from the transcript of the gene encoding yeast alanine tRNA. Determination of the 77 nucleotide sequence required 1 g of alanine tRNA, recovered from. 200 g of bulk tRNA, isolated from 140 kg
openaire   +1 more source

Transcriptome Sequencing: RNA-Seq

2018
RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of ...
Hong, Zhang, Lin, He, Lei, Cai
openaire   +2 more sources

RNA Sequencing Protocols for Short-Read Sequencing

RNA sequencing (RNA-seq) methodologies allow the discovery of novel variants and transcripts. These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform.
Laura, Vasquez-Velez, Veera, D'Mello, Patricia, Soteropoulos   +2 more
openaire   +2 more sources

Nuclear RNA Isolation and Sequencing

2016
Most transcriptome studies involve sequencing and quantification of steady-state mRNA by isolating and sequencing poly (A) RNA. Although this type of sequencing data is informative to determine steady-state mRNA levels it does not provide information on transcriptional output and thus may not always reflect changes in transcriptional regulation of gene
Navroop K, Dhaliwal, Jennifer A, Mitchell   +1 more
openaire   +3 more sources

Aligning Multiple RNA Sequences

1991
Each cell in a living organism contains a complete design for all of the mechanisms that sustain its life. This blueprint is encoded in DNA and is called the genome of the organism. The basic activity of the cell involves maintaining an environment in which appropriate concentrations of specific proteins drive the reactions that characterize its normal
Ross A. Overbeek, Ian T. Foster
openaire   +1 more source

Normalized Similarity of RNA Sequences

2005
We introduce a normalized version of the LCS metric as a new local similarity measure for comparing two RNAs. An $\mathcal{O}(n^{2}m{\rm lg}m)$ time algorithm is presented for computing the maximum normalized score of two RNA sequences, where n and m are the lengths of the sequences and n ≤ m.
Rolf Backofen, Danny Hermelin, Gad M. Landau, Oren Weimann   +3 more
openaire   +2 more sources