The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool [PDF]
, 2018 Background: Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion.
de Vrieze, Jo +3 more
core +2 more sources
Metagenomes, metatranscriptomes and microbiomes of naturally decomposing deadwood
Scientific Data, 2021 Measurement(s) metagenomic data • metatranscriptomic data • microbiome • RNA-seq of total RNA Technology Type(s) DNA sequencing • RNA-seq of total RNA • amplicon sequencing • RNA sequencing Factor Type(s) time of decomposition Sample Characteristic ...
Vojtěch Tláskal +7 more
doaj +1 more source
Small RNA transcriptome analysis using parallel single-cell small RNA sequencing
Scientific Reports, 2023 miRNA and other forms of small RNAs are known to regulate many biological processes. Single-cell small RNA sequencing can be used to profile small RNAs of individual cells; however, limitations of efficiency and scale prevent its widespread application ...
Jia Li +4 more
doaj +1 more source
A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]
, 2012 With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood +8 more
core +2 more sources
Integrative analysis revealed the molecular mechanism underlying RBM10‐mediated splicing regulation
EMBO Molecular Medicine, 2013 RBM10 encodes an RNA binding protein. Mutations in RBM10 are known to cause multiple congenital anomaly syndrome in male humans, the TARP syndrome. However, the molecular function of RBM10 is unknown.
Yongbo Wang +14 more
doaj +1 more source
A comparison between whole transcript and 3' RNA sequencing methods using Kapa and Lexogen library preparation methods. [PDF]
, 2019 Background3' RNA sequencing provides an alternative to whole transcript analysis. However, we do not know a priori the relative advantage of each method.
Fuqua, Brie K +6 more
core +1 more source
G3: Genes, Genomes, Genetics, 2021 RNA sequencing (RNA-Seq) experiments focused on gene expression involve removal of ribosomal RNA (rRNA) because it is the major RNA constituent of cells.
Calla L Telzrow +7 more
doaj +1 more source
YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs. [PDF]
, 2017 Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles.
Honda, Shozo +5 more
core +2 more sources
Native RNA or cDNA Sequencing for Transcriptomic Analysis: A Case Study on Saccharomyces cerevisiae
Frontiers in Bioengineering and Biotechnology, 2022 Direct sequencing of single molecules through nanopores allows for accurate quantification and full-length characterization of native RNA or complementary DNA (cDNA) without amplification.
Thidathip Wongsurawat +3 more
doaj +1 more source
Complete Genome Sequence of Diaphorina citri-associated C virus, a Novel Putative RNA Virus of the Asian Citrus Psyllid, Diaphorina citri. [PDF]
, 2016 We present here the complete nucleotide sequence and genome organization of a novel putative RNA virus identified in field populations of the Asian citrus psyllid, Diaphorina citri, through sequencing of the transcriptome followed by reverse ...
Falk, Bryce W +2 more
core +1 more source