Results 31 to 40 of about 1,474,432 (298)
Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]
, 2013 Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core +12 more sources
Biological Procedures Online, 2023 Background RNA sequencing has become the gold standard for transcriptome analysis but has an inherent limitation of challenging quantification of low-abundant transcripts.
Celine Everaert +6 more
doaj +1 more source
RNA-Seq optimization with eQTL gold standards. [PDF]
, 2013 BackgroundRNA-Sequencing (RNA-Seq) experiments have been optimized for library preparation, mapping, and gene expression estimation. These methods, however, have revealed weaknesses in the next stages of analysis of differential expression, with results ...
Arking, Dan E +5 more
core +2 more sources
Comparison of DNA and RNA sequencing of total nucleic acids from human cervix for metagenomics
Scientific Reports, 2021 Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare these strategies.
Laila Sara Arroyo Mühr +4 more
doaj +1 more source
Single-Cell RNA Sequencing in Parkinson’s Disease
Biomedicines, 2021 Single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) technologies have enhanced the understanding of the molecular pathogenesis of neurodegenerative disorders, including Parkinson’s disease (PD).
Shi-Xun Ma, Su Bin Lim
doaj +1 more source
FLASH: ultra-fast protocol to identify RNA-protein interactions in cells
, 2019 Determination of the in vivo binding sites of RNA-binding proteins (RBPs) is paramount to understanding their function and how they affect different aspects of gene regulation.
Akhtar, A. +4 more
core +1 more source
Advancing transcriptome platforms [PDF]
, 2011 During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
core +2 more sources
Getting the Entire Message: Progress in Isoform Sequencing
Frontiers in Genetics, 2019 The advent of second-generation sequencing and its application to RNA sequencing have revolutionized the field of genomics by allowing quantification of gene expression, as well as the definition of transcription start/end sites, exons, splice sites and ...
Simon A. Hardwick +5 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2023 Low back pain (LBP) is a prevalent health problem worldwide that affects over 80% of adults during their lifetime. Intervertebral disc degeneration (IDD) is a well-recognized leading cause of LBP.
Xiao Yang +4 more
doaj +1 more source
Single-cell RNA sequencing in orthopedic research
Bone Research, 2023 Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid ...
Tao Wang +5 more
doaj +1 more source